摘要
慢性粒细胞白血病(chronic myeloid leukemia,CML)是造血干细胞(hematopoietic stem cells,HSC)恶性克隆性增殖引起的一种血液系统疾病。动物模型是研究CML发病机制及药物靶向治疗的重要载体和工具。研究表明,CML小鼠模型可以通过逆转录病毒介导、转基因和白血病细胞移植的方法建立。三种方法建立的CML小鼠模型均可用于CML发病机制及药物疗效评估研究。实验动物模型进一步通过血常规、血涂片和骨髓涂片、免疫学、分子生物学及病理学等检测手段,判断模型是否建立成功。本文就近年来CML小鼠模型的建立、鉴定及研究应用进展进行综述。
Chronic myeloid leukemia(CML) is a blood system disease caused by malignant clonal proliferation of hematopoietic stem cells(HSCs). Animal models are important tools for studying the pathogenesis of CML and drug-targeted therapy research. Studies have shown that the CML mouse model can be established by retroviral-mediated, transgenic and leukemia cell transplantation methods.The CML mouse model established by the three methods can be used to research and unravel the pathogenesis mechanism of CML, and to evaluate CML drug efficacy of therapy. The experimental animal model further judges whether the model is successfully established by means of blood routine, blood smear and bone marrow smear, immunology, molecular biology and pathology. In this paper, the advances of the establishment, identification and application of CML mouse models in recent years are reviewed.
作者
武小亮
卢林纲
王靖
任广旭
韩娟
WU Xiao-liang;LU Lin-gang;WANG Jing;REN Guang-xu;HAN Juan(Food and nutrition assessment laboratory,Institute of food and nutrition development,Ministry of Agriculture and Rural Affairs,Beijing,100081,China)
出处
《现代生物医学进展》
CAS
2019年第1期197-200,共4页
Progress in Modern Biomedicine
基金
中国农业科学院创新工程项目(CAAS ASTIP32017IFND)
农业部食物与营养发展研究所基本科研业务费专项(1610422017007)
