摘要
基因敲除技术是20世纪80年代发展起来的一种分子生物学技术,目前已广泛应用于微生物基因功能等相关领域的研究中。维氏气单胞菌(Aeromonas veronii,A. veronii)是气单胞菌科气单胞菌属的一种,近年来,该菌引发疾病的报道逐年增多,因此,对其致病机制的研究显得尤为重要。本文对细菌不同基因敲除策略及其在A. veronii基因功能研究中的应用作一综述,旨在为A. veronii致病机制的深入研究提供参考。
Gene knockout is a molecular biological technique developed in 1980 s,which has been widely used for the studies in relevant fields such as gene function of microorganism.The diseases caused by Aeromonas veronii increased year by year,thus it is important to study the pathogenic mechanism.This paper reviews the application of various gene knockout strategies in the study on gene function of A.veronii in order to provide a reference for further study on pathogenic mechanism of the bacterium.
作者
康元环
杨滨僮
单晓枫
钱爱东(校)
KANG Yuan-huan;YANG Bin-tong;SHAN Xiao-feng;QIAN Ai-dong(College of Animal Science and Technology,Jilin Provincial Engineering Research Center of Animal Probiotics,Key Laboratory for Animal Production,Product Quality and Safety of Ministry of Education,China,Jilin Agricultural University,Changchun 130118,Jilin Province,China)
出处
《中国生物制品学杂志》
CAS
CSCD
2019年第6期698-702,共5页
Chinese Journal of Biologicals
基金
吉林省教育厅“十三五”科学技术项目(JJKH20190910KJ)
国家自然科学基金项目(31201927)
吉林省重点科技攻关项目(20150204065NY)
吉林省科技厅自然基金项目(20170101016JC)
