摘要
目的观察电针'水沟''内关''三阴交''委中'穴,对脑缺血再灌注大鼠缺血半暗带区胶质细胞源性神经营养因子(glial cell line derived neurotrophic factor,GDNF)表达的影响,探讨其减轻脑缺血再灌注损伤的作用机制。方法将120只清洁级成年雄性Sprague-Dawley(SD)大鼠,随机分为空白组、假手术组、模型组、电针组和抑制剂组5组,每组24只。参照Longa改良线栓法,制备大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型。电针组以电针刺激大鼠'水沟''内关''三阴交''委中'穴,每天1次,连续治疗28天。抑制剂组以NF-κB抑制剂PDTC进行大鼠腹腔注射后(120mg/kg),再行电针刺激,每天1次,连续治疗28天。应用实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR),检测大鼠脑组织缺血半暗带区GDNF的mRNA表达。结果与模型组相比,电针组GDNFmRNA表达在治疗后第3天时下降,在第7、14、28天时表达上升,差异有统计学意义(P<0.05);NF-κB抑制剂组GDNFmRNA表达在第3、7天时下降(P<0.05),在第14、28天时差异不明显无统计学意义(P>0.05)。与电针组比较,抑制剂组在治疗后4个观察时间点GDNFmRNA表达均下调明显,差异有统计学意义(P<0.05)。结论电针治疗能上调GDNFmRNA的表达,对缺血再灌注损伤大鼠起到脑保护作用,其作用机制可能与NF-κB信号通路的活跃性有关。
Objective To observe the effect of electro-acupuncture at Shuigou,Neiguan,Sanyinjiao and Weizhong points on the expression of glial cell line derived neurotrophic factor(GDNF)in ischemic penumbra of rats with cerebral ischemia-reperfusion,and to explore its mechanism of reducing cerebral ischemia-reperfusion injury.Methods 120 adult male Sprague-Dawley(SD)rats of clean grade were randomly divided into five groups:blank group,sham operation group,model group,electro-acupuncture group and inhibitor group,with 24 rats in each group.The middle cerebral artery occlusion(MCAO)model was established by using Longa’s modified thread embolization method.In the electro-acupuncture group,rats were stimulated by electro-acupuncture at Shuigou,Neiguan,Sanyinjiao and Weizhong points once a day for 28 days.The inhibitor group was intraperitoneally injected with NF-κB inhibitor PDTC(120 mg/kg),and then electro-acupuncture was stimulated once a day for 28 days.Real-time fluorescence quantitative polymerase chain reaction(PCR)was used to detect the mRNA expression of GDNF in the ischemic penumbra of rat brain.Results Compared with the model group,the expression of GDNF mRNA in the electroacupuncture group decreased on the 3 rd day after treatment,and the expression increased on the 7 th,14 th and 28 th days,the difference was statistically significant(P<0.05).The expression of GDNF in NF-kappa B inhibitor group decreased on the 3 rd and 7 th day(P<0.05),but there was no significant difference on the 14 th and 28 th day(P>0.05).The expression of GDNF mRNA was significantly down-regulated in the inhibitor group at the four observation time points after treatment,and the difference was statistically significant(P<0.05).Conclusion Electroacupuncture can up-regulate the expression of GDNFmRNA and protect the rats from ischemia-reperfusion.The mechanism may be related to the activity of NF-kB signaling pathway.
作者
刘霜月
许潇莹
张玉莲
艾双春
Liu Shuangyue;Xu Xiaoying;Zhang Yulian;Ai Shuangchun(Chengdu University of Chinese Medicine,Chengdu China,610075;Mianyang TCM Hospital,Mianyang China,621000)
出处
《陕西中医药大学学报》
2019年第5期58-63,共6页
Journal of Shaanxi University of Chinese Medicine
基金
绵阳市科技局资助项目(2015yy037)
