摘要
目的:检测EZH2和SMYD3蛋白在结直肠癌组织中的表达,分析EZH2和SMYD3蛋白的表达情况与结直肠癌不同病理特征及预后的相关性,探讨EZH2和SMYD3蛋白在结直肠癌发生发展中作用相关性,为结直肠癌的诊断、治疗及预后判断提供全新的分子标志及靶点。方法:结直肠癌组织(实验组)及癌旁组织(对照组)标本取自郑州大学附属肿瘤医院普外科结直肠癌患者根治术后石蜡包埋标本,共60例。采用免疫组织化学方法检测结直肠癌组织及癌旁组织中EZH2和SMYD3蛋白表达情况,结合结直肠癌患者的术后病理,根据2017版NCCN指南完成TNM分期,用χ~2检验分析EZH2和SMYD3蛋白在结直肠癌组织及癌旁组织中的表达差异,分析EZH2和SMYD3蛋白表达情况与结直肠癌术后病理参数之间的关系;用Kaplan-Meier法、Log-rank检验分析EZH2和SMYD3蛋白与结直肠癌患者根治术后无病生存期(DFS)及总生存期(OS)之间的关系,采用Pearson相关性分析EZH2和SMYD3蛋白在结直肠癌组织中表达的相关性。结果:EZH2及SMYD3蛋白在结直肠癌组织中的阳性率明显高于癌旁组织,表明两者均在结直肠癌的发展中表达上调。EZH2蛋白表达情况与肿瘤TNM分期、有无淋巴结转移有显著相关性,SMYD3蛋白表达情况与肿瘤TNM分期、有无淋巴结转移、肿瘤发生部位有显著相关性,表明两者的表达可能与结直肠癌的侵袭和转移有关。EZH2、SMYD3蛋白表达阳性结直肠癌患者术后DFS较短,表明两者的表达可能提示结直肠癌患者预后不良。在结直肠癌组织中,EZH2、SMYD3蛋白的表达情况呈正相关关系,表明两者在结直肠癌的发生发展过程中可能呈协同作用。结论:EZH2、SMYD3蛋白在结直肠癌组织中的表达上调,在结直肠癌组织表达中呈明显正相关,并且与结直肠癌的不良预后相关,可能会是结直肠癌患者重要的预后因子及治疗靶点。
Objective:To detect the expression of EZH2 and SMYD3 protein in colorectal cancer tissues,analyze the expression of EZH2 and SMYD3 protein and their correlation with different pathological features and prognosis of colorectal cancer.To investigate the role of EZH2 and SMYD3 proteins in the development of colorectal cancer and to provide new molecular markers and targets for the diagnosis,treatment and prognosis of colorectal cancer.Methods:Colorectal cancer tissues(experimental group) and paracancer tissues(control group) were collected from paraffin-embedded specimens of patients undergoing radical resection of colorectal cancer at the Department of General Surgery,Affiliated Tumor Hospital of Zhengzhou University.Altogether 60 cases were included.Immunohistochemistry was used to detect the expression of EZH2 and SMYD3 protein in colorectal cancer tissues and adjacent tissues.Combining the postoperative pathology of patients with colorectal cancer,the TNM staging was completed according to the 2017 edition of the NCCN guidelines.The χ~2 test was used to analyze the expression differences of EZH2 and SMYD3 proteins in colorectal cancer tissues and adjacent tissues.The relationship between the expression of EZH2 and SMYD3 protein and pathological parameters of colorectal cancer was analysed.Kaplan-Meier method and Log-rank test were used to analyze the relationship between EZH2 and SMYD3 protein and disease-free survival(DFS) and overall survival(OS) after radical resection in patients with colorectal cancer.Pearson correlation was used to analyze the correlation of EZH2 and SMYD3 protein expression in colorectal cancer tissues.Results:The positive rate of EZH2 and SMYD3 protein in colorectal cancer tissue was significantly higher than that in paracancer tissue,indicating that both of them were up-regulated in the development of colorectal cancer.The expression of EZH2 protein was significantly correlated with TNM stage and lymph node metastasis.The expression of SMYD3 protein was significantly correlated with TNM stage,lymph node metastasis and tumor site,indicating that the expression of both may be related to the invasion and metastasis of colorectal cancer.Patients with EZH2,SMYD3 protein positive colorectal cancer had a shorter postoperative DFS,suggesting that both twoexpression may indicate poor prognosis in patients with colorectal cancer.In colorectal cancer tissues,there was a positive correlation between the expression of EZH2 and SMYD3 proteins,indicating that they may have a synergistic effect in the development of colorectal cancer.Conclusion:EZH2 and SMYD3 protein are highly expressed in colorectal cancer tissue,and the expression has significantly positive correlation. They may be associated with poor prognosis of colorectal cancer,and may be important prognostic factors of patients with colorectal cancer and therapeutic targets.
作者
李燕
谢晋玲
邓文英
李宁
罗素霞
Li Yan;Xie Jinling;Deng Wenying;Li Ning;Luo Suxia(The Affiliated Cancer Hospital of Zhengzhou University,Henan Zhengzhou 450000,China)
出处
《现代肿瘤医学》
CAS
2019年第8期1366-1371,共6页
Journal of Modern Oncology
基金
河南省科技攻关项目(编号:152102310158)
