摘要
目的探讨流式细胞术(FCM)计数血小板的应用价值。方法用流式细胞术与电阻抗法计数血小板并与(参考方法)手工法进行比较。结果 3种方法计数血小板均有相关性(P<0.01),血小板计数在(1～300)×10~9/L相关系数>0.97,血小板计数在(1～100)×10~9/L较低范围时,相关系数(r)>0.95。与参考方法比较,血小板计数>100×10~9/L时2种方法的相关系数分别为0.98、0.97,血小板<100×10~9/L时,相关系数为0.95、0.86。结论 FCM计数血小板优于电阻抗法,特别对血小板减少症的患者。
Objective To study the clinical application of platelet(PLT)count by flow cytometric method(FCM).Methods PLT count was calculated by FCM and impedance method,compared with reference method(manual method).Results Three kinds of method for PLT count correlated extremely well(P<0.01).When PLT was(1-300)×10~9/L,correlative coefficient was over 0.97.When PLT was(1-100)×10~9/L,correlative coefficient was over 0.95.Comparison of reference method with manual method,when PLT was over 100×10~9/L,correlative coefficient of two methods was over 0.98 and 0.97;When PLT was below 100×10~9/L,correlative coefficient of two methods were over 0.95 and 0.86.Conclusion It suggests that flow cytometric methods of PLT count might be more accurate than impedance method,especially for patients with thrombocytopenia.
作者
黎庆梅
梁彩云
江雪霞
高凯
麦惠霞
吴昊
Li Qingmei;Liang Caiyun;Jiang Xuexia;Gao Kai;Mai Huixia;Wu Hao(Department of AIDS Prevention and Control,Guangzhou Center for Disease Control and Prevention,Guangdong 510440,China)
出处
《实用医技杂志》
2019年第4期416-417,共2页
Journal of Practical Medical Techniques
