摘要
目的研究盐胁迫下接种短小芽孢杆菌Bacillus pumilus对甘草生长特性及抗氧化能力的影响。方法通过水培发芽试验,采用完全随机设计,处理分别为对照(CK)、盐胁迫(NaCl)、对照加菌(CK+B)、盐胁迫加菌(NaCl+B)。结果与对照相比,盐胁迫使得甘草的胚芽、胚根粗度及干重减少,胚根长度增加。盐胁迫下接种短小芽孢杆菌后,甘草的胚芽粗度、干重增加,胚根长度减少。与对照相比,盐胁迫处理降低甘草中SOD、CAT和GPX活性,使得甘草中O_2^-产生速率及H_2O_2和MDA含量增加,而接种短小芽孢杆菌提高了甘草中SOD、POD和CAT活性,从而降低了O_2^-产生速率、H_2O_2和MDA含量。结论接种短小芽孢杆菌通过提高抗氧化酶活性、降低活性氧,缓解盐胁迫造成的氧化损伤,进而提高甘草的生长。
Objective To study the effects of Bacillus pumilus inoculation on the growth characteristics and antioxidant capacity of Glycyrrhiza uralensis under salt stress.Methods The hydroponic germination test was carried out.The treatment was CK,NaCl,CK+B and NaCl+B respectively.Results Compared with the control,salt stress reduced the diameter and dry weight of radicle,and increased the length of radicle.After inoculation with Bacillus pumilus under salt stress,the germ diameter,dry weight and radicle length of Glycyrrhiza uralensis increased.Compared with the control,salt stress decreased the activity of SOD,CAT and GPX,increased the production rate of O2-and the content of H2O2 and MDA,while inoculation with Bacillus pumilus increased the activity of SOD,POD and CAT,and decreased the production rate of O2-and the content of H2O2 and MDA.Conclusion Inoculation of Bacillus pumilus can alleviate the oxidative damage caused by salt stress by increasing the activity of antioxidant enzymes and reducing reactive oxygen species,and then improve the growth of Glycyrrhiza uralensis.
作者
张晓佳
解植彩
张文晋
余霞霞
张瑜
崔高畅
郎多勇
王建寰
张新慧
ZHANG Xiao-jia;XIE Zhi-Cai;ZHANG Wen-jin;YU Xia-xia;ZHANG Yu;CUI Gao-chang;LANG Duo-yong;WANG Jian-huan;ZHANG Xin-hui(School of Pharmacy,Ningxia Medical University,Yinchuan 750004,China;Ningxia Hui Medicine Modern Engineering Technology Research Center/Ningxia Hui Medicine Cooperative Innovation Center/Key Laboratory of Hui Medicine Modernization Education,Yinchuan 750004,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2019年第3期688-691,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(31460330
318603437)
国家重点研发计划中医药现代化研究(2017YFC1700706)
关键词
甘草
盐胁迫
短小牙孢杆菌
抗氧化
生长特性
Glycyrrhiza uralensis Fisch.
Salt stress
Bacillus pumilus
Antioxidant capacity
Growth characteristics
