摘要
目的 :研究K+ 通道在Jurkat细胞调节性体积减小 (RVD)中的作用。方法 :调节细胞外不同离子浓度 ,用细胞成像系统测定Jurkat细胞 (人T淋巴细胞肿瘤细胞株 )在不同渗透压时细胞容积的变化。结果 :细胞培养液中缺少Ca2 + ,或细胞培养液中的K+ 浓度升高 ,或使用Ca2 + 敏感的K+ 通道 (KCa)阻断剂均可抑制Jurkat细胞的RVD ;应激免疫抑制蛋白 (ISPS)具有抑制Jurkat细胞RVD的作用。结论 :Jurkat细胞中KCa通道是RVD不可缺少的因素。ISPS对免疫功能抑制作用的机理之一 ,可能是抑制了T淋巴细胞上的K+
AIM: To study the effect of K + channel on the regulatory volume decrease (RVD) in Jurkat cells. METHODS: An imaging analysis system was used for the measurement of cell volume under the conditions of hypotonic stress in Jurkat cells. RESULTS: RVD in Jurkat cells was found to be inhibited under the conditions of lack of Ca 2+ in the medium, and increased in concentration of K + or administration of KCa channel blocker. Immune suppressive protein of stress (ISPS) was also able to inhibit the RVD in Jurkat cells. CONCLUSION: KCa channel plays an important role in RVD in Jurkat cells. The suppression on K + channel may be one of the possible reasons for the inhibition on immune function induced by ISPS.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2003年第5期499-502,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金项目 (№ 30 2 712 0 9)
