摘要
目的 :观察大鼠缺血心肌细胞核钙转运功能的变化及川芎嗪的作用。方法 :将Wistar大鼠随机分成对照组、缺血组和川芎嗪保护组。采用皮下注射异丙肾上腺素 (ISP,5mg·kg- 1 ) ,2 4h后再注射相同剂量一次 ,造成心肌缺血模型。超速离心纯化细胞核 ,酶学法鉴定核纯度和测定核膜Ca2 + ATPase活性 ,同位素法观测核钙的摄取。结果 :缺血组心肌细胞核膜Ca2 + ATPase活性较对照组下降 2 1 .4% (P<0 .0 1 ) ,4 5Ca2 + 摄取率也呈显著性降低 ,其最大反应速度 (Vmax)降低 48.5 % ,细胞核膜Ca2 + 依赖性ATP酶的Vmax 降低 3 4.1 % ,Km 值降低 42 .2 % (P <0 .0 1 ) ,川芎嗪保护组心肌细胞核Ca2 + ATP酶活性及核4 5Ca2 + 摄取率较缺血组均有显著性升高 ,且有剂量依赖关系。结论
AIM: To observe the effects of ligustrazin on dysfunction of myocardial nuclear calcium transport in ischemic myocardial cells of rats. METHODS: The ischemic models of myocardial cells were induced by subcutaneous injection of isoproterenol (ISP,5 mg·kg -1 ·d -1 ). Myocardial nuclei were purified with ultracentrifugation and identified by enzymological assay. The activity of Ca 2+ ATPase was measured by the same way and calcium uptake was assayed with 45 Ca 2+ isotope. RESULTS: The Ca 2+ ATPase activity of myocardial nuclear membrane in ischemia group was decreased by 21.4 % (P< 0.05 ) in comparison with that in control group. The nuclear 45 Ca 2+ uptake significantly decreased, and V max value also decreased by 48.5 %. V max and K m of ATPase decreased by about 34.1 % (P< 0.05 ) and 42.2 % (P< 0.01 ) respectively. Ca 2+ ATPase activity and 45 Ca 2+ uptake in the myocardial nuclei of the ligustrazin group showed no significant change. CONCLUSION: Ligustrazin has notably protective effects on dysfunction of nuclear calcium transport in myocardial cells of rats.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2003年第5期588-590,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
