摘要
目的 研究阿克拉霉素 (ACM)体外抑制原代急性白血病 (AL)细胞的生长及其机理。方法 MTT法研究 37例初发AL细胞的生长抑制。DNA片段原位末端标记 (TUNEL)法检测 2 0例AL细胞的凋亡率。结果 ACM体外能明显抑制原代AL细胞的生长 ;细胞形态学、DNA琼脂糖凝胶电泳均证实ACM能诱导原代AL细胞凋亡 ;与空白对照相比 ,ACM在体外与原代AL细胞共同培养 15小时后 ,TUNEL阳性细胞率明显增高 ,差异有统计学意义 (30 89± 15 90 %对 14 85± 15 90 %;P <0 0 1) ;且TUNEL阳性细胞率与MTT抑制率呈正相关 (r =0 32 6 ,P =0 0 4)。结论 诱导细胞凋亡是ACM抑制原代AL细胞增殖的重要机制之一。
Objective To elucidate the proliferation inhibition induced by aclacinomycin(ACM) in vitro and its mechanism.Methods The proliferation inhibition of leukemic cells of 37 patients with AL was examined by MTT assay.The apoptosis of leukemic cells of 20 patients with AL was analyzed with the method of TUNEL (terminal deoxyribonucleotidyl transferase-mediated dUTP-biotin nick end labeling).Results Cytostatic effect induced by ACM was significant;The apoptosis of leukemic cells induced by ACM was confirmed from the change of cell morphology and DNA agar glucose electroresis;when AL cells were cultured with ACM for 15 hours,the rate of TUNEL-positive AL cells was significantly higher than that in the control group(30.89±15.90% vs 14.85±15.90%,P<0.01);And it was correlated colsely with the TUNEL-positive rate and MTT proliferation inhibition of AL cells induced by ACM(r=0.326,P=0.04).Conclusions These findings suggested that ACM can induce apoptosis of primary AL cells,that may be one of the mechenisms of ACM-induced proliferation inhibition of leukemic cells.
出处
《河南肿瘤学杂志》
2003年第4期237-239,共3页
Henan Journal of Oncology
