摘要
①目的 探讨小剂量多巴胺对急性出血坏死性胰腺炎 (AHNP)的治疗作用。②方法 将Wistar大鼠5 0只随机分为两组 ,向大鼠胰管注射牛磺胆酸钠建立AHNP动物模型。多巴胺治疗组 (n =2 5 )静脉给予 5 μg/(kg·min)多巴胺 ,对照组 (n =2 5 )静脉给予生理盐水 ,于实验前和实验后 2、4、6、12、2 4h经颈静脉取血 ,采用双抗体夹心ELISA法测定血清中白细胞介素 6 (IL 6 )、肿瘤坏死因子α(TNF α)水平。术后 2 4h处死大鼠 ,取胰腺组织行病理检查。③结果 AHNP形成后治疗组血清IL 6、TNF α水平均较对照组低 ,差异有显著意义 (t=6 .2 3～ 32 .32 ,P <0 .0 1) ;治疗 2 4h时 ,治疗组血清IL 6、TNF α水平与AHNP形成前差异无显著性 (t =1.99、1.71,P >0 .0 5 )。胰腺病理组织学评分治疗组明显低于对照组 ,差异有显著性 (t=3.92～ 9.94 ,P <0 .0 1)。④结论 小剂量多巴胺可减轻AH NP的炎症反应 。
Objective\ To study the treatment of acute hemorrhagic necrotic pancreatitis(AHNP) with low dose dopamine.\ Methods\ Fifty Wistar rats were divided into two groups, treatment group and control group. An animal model of acute pancreatitis was established by injecting sodium taurocholate into the pancreatic duct. Treatment group ( n =25) : 5 μg/kg was given intravenously while for control one ( n =25), normal saline was given. Blood samples were taken from the jugular vein at 2, 4, 6, 12 and 24 hours after the experiment. The IL 6 and TNF α were detected by ELISA technique. The pancreas was examined histologically 24 hours after the treatment. \ Results\ The serum levels of IL 6 and TNF α of treatment group were lower than that of the control one, the difference was significant ( t=6.23-32.32, P <0.01). After 24 hours of the treatment, the difference of IL 6 and TNF α was not significant as compared with that before the formation of AHNP ( t=1.99, 1.71; P >0.05). The grade of pancreatic histopathology of dopamine group was obviously lower than that of the control group. \ Conclusion\ Low dose dopamine may reduce the inflammatory reaction of acute pancreatitis, which may be something to do with that low dose of dopamine not only increase the blood flow of pancreatic micrangium but also reduce its permeability.
出处
《青岛大学医学院学报》
CAS
2003年第3期242-243,245,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
青岛市科学技术局资助项目 (ZD0 1 0 88)
