摘要
①目的 界定大鼠局灶性脑缺血再灌注后脑缺血半影区的范围。②方法 应用线栓法建立大鼠大脑中动脉闭塞再灌注模型 ,氯化三苯基四氮唑 (TTC)染色、甲苯胺蓝染色、焦油紫染色和苏木精 伊红染色界定脑缺血半影区和中心区。③结果 脑缺血再灌注 2h ,缺血病灶位于纹状体外侧区和额顶叶皮质下部 ,随着缺血再灌注时间的延长病灶范围逐渐扩大 ;再灌注 1～ 2d最大 ,波及纹状体内侧区和额顶叶皮质上部以及梨状区皮质 ,之后逐渐缩小 ;再灌注 7～ 14d恢复到再灌流 2h的水平。缺血病灶与周围正常脑组织之间有一形态和结构过渡区 ,该区的着色和细胞形态结构界于病灶和正常脑组织之间 ,即缺血半影区。缺血中心区神经细胞明显减少 ,呈现坏死特征 ;缺血半影区细胞主要呈现核固缩、染色质凝聚等凋亡特征。④结论 脑缺血再灌注缺血中心区位于纹状体外侧区和额顶叶皮质下部 ,缺血半影区位于纹状体内侧区和额顶叶皮质上部以及梨状区皮质。
Objective\ To define the histologic range of cerebral ischemic penumbra after focal cerebral ischemia reperfusion in rats.\ Methods\ A model of middle cerebral artery occlusion/reperfusion w as established. The ischemic penumbra and central area were distinguished by TTC , Toluidine blue, Nissl and HE staining methods. \ Results\ The ischemic area located at lower frontoparietal cortex and lateral striatum 2 h after reperfusion, then increased gradually and peaked on 1-2 d and expanded to the upper frontoparietal cortex, piriform cortex and me dial striatum, and returned to reperfusion of 2 h level on day 7-14. There was a transitional area in which the staining color and the structure of neuron wer e between normal and ischemic area. The number of neuron reduced significantly w ith main characteristics of necrosis in ischemic central area and apoptotic prop erty in ischemic penumbra. \ Conclusion\ The ischemic central area located at lower frontoparie tal cortex and lateral striatum, while the ischemic penumbra at upper frontopari etal cortex, medial striatum and piriform cortex.
出处
《青岛大学医学院学报》
CAS
2003年第4期370-371,374,F002,共4页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省自然科学基金资助项目 (编号Y2 0 0 1C0 5 )
