摘要
目的:观察年龄对成年小鼠嗅球细胞增殖与神经发生的影响。方法:用5-溴-2-脱氧尿核苷(5-bromo-2’-deoxyuridine,BrdU)标记新生细胞,免疫组化染色后统计新生细胞数量;应用BrdU与TuJ1/GFAP双标记,鉴别出BrdU阳性细胞的细胞类型。结果:老年组小鼠嗅球细胞增殖与神经发生明显低于青年组。结论:成年小鼠嗅球的细胞增殖与神经发生随着年龄的增长而减慢,这可能是嗅觉减退的结构基础之一。
Objective:To study the effects of age on cell proliferation and neurogenesis in the adult mouse olfactory bulb.Methods:Proliferating cells were labeled with5-bromo-2'-deoxyuridine(BrdU)for10days.BrdU immunohistochemistry was conducted to dertermine the number of newly generated cells and double immunolabeling undertaken to co-localize BrdU-positive cells with TuJ1(Tubulin-βAb-3,neu-ron-specific early differentiation marker)/GFAP(rabbit anti-glial fibrillary acid protein).Results:The number of new-born cells in the aged mice was significantly lower than that in the young mice.Conclu-sion:Cell proliferation and neurogenesis in the olfactory bulb decrease with the age increase of the adult mice,which may be related to the decrease of odor discrimination in the aged.
出处
《山东大学学报（医学版）》
CAS
2003年第4期365-367,共3页
Journal of Shandong University:Health Sciences
关键词
溴脱氧尿苷
嗅球
细胞增殖
神经发生
年龄组
小鼠
Bromodeoxyuridine
Olfactory bulb
Cell proliferation
Neurogenesis
Age groups
Mice
