摘要
目的 :改进红参中人参皂苷Rg1和人参皂苷Re的含量测定方法。方法 :高效液相色谱法。色谱柱为C1 8柱 ,流动相为乙腈 水 (2 0∶80 ) ,检测波长为 2 0 3nm。结果 :人参皂苷Rg1和人参皂苷Re的线性范围分别为 0 6 0 0 8～ 6 0 0 80 μg和 0 4 0 32～4 0 32 0 μg;平均回收率分别为 98 5 % (RSD =1 3% ,n =6 )和 99 2 % (RSD =1 1% ,n =6 )。结论 :本法简单、快速 ,结果准确。
Objective:To improve on the HPLC method for determination of Ginsenosid Rg1 and Ginsenoside Re in Hongshen (Red Ginsen).Method:A C 18 column was used.The mobile phase consisted of CH-3CN-H-2O(20∶80) and the detection wavelength was 203nm.Results:The calibration curves were separately linear of Ginsenoside Rg1 and Ginsenoside Re between 0^6008～6^0080μg,0^4032～4.0320 μg;the average recoveries of Ginsenoside Rg1 and Ginsenoside Re were separately 98^5%(RSD1^3%,n6) and 99^2%(RSD1^1%,n6).Conclusion:This method is simple,quick,reproducible and is suitable for the quality control of Hongshen.
出处
《中国实验方剂学杂志》
CAS
2003年第5期10-11,共2页
Chinese Journal of Experimental Traditional Medical Formulae
