摘要
目的 :探讨家兔被脑膜炎双球菌 (MC)攻击后脾组织的病变特征。方法 :模型组(n =10 )耳缘静脉注射MC A群菌株 (按 1× 10 11菌体·kg-1的剂量 )复制成动物模型 ,对照组(n =8)予以等量的生理盐水。给药前及给药后 60、12 0min分别抽血检测血浆肿瘤坏死因子 α(TNF α)浓度 ,12 0min后处死动物 ,取脾作TNF α细胞组织化学和超微结构检查。结果 :模型组白髓内炎性及损伤的细胞周围有TNF α沉积 ,脾索、脾窦、红细胞边缘也有TNF α沉积 ,红细胞结构破坏 ,血浆TNF α水平增高。对照组无异常。结论 :MC攻击家兔后可导致TNF
Objective To explore the pathologic characteristics of spleen of rabbit infected with meningococci (MC).Methods New Zealand rabbits were injected with MC(1×10 11 organism·kg -1 ,model group, n =10) iv,or normal saline (control group, n =8).TNF α levels of plasm were detected pre?administration 60?min and 120?min after administration respectively.At 120?min,the rabbits were sacrificed,and their spleens were examined histochemically and morphologically under electron microscope.Results Immunoreaction was localized in peri?inflammatory cells in white pulp, and it also localized in splenic cord,sinusoid and pre?red cells.TNF α levels of plasm were elevated during 120?min in model group.Conclusion MC infection in rabbits results in TNF α release in their spleens which in turn causes spleen damage.
出处
《东南大学学报（医学版）》
CAS
2003年第5期305-308,共4页
Journal of Southeast University(Medical Science Edition)
基金
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