摘要
采用门控进样 ,在简单的十字通道微流控玻璃芯片上实现了假随机多次进样 ,研究了利用哈达玛变换提高微流控毛细管电泳分析系统信噪比的方法 .在实验中 ,以 7阶 1 2 7步假随机二进制序列作为进样模板 ,将缓冲液和 Cy5衍生后的氨基酸试样交替注入到分离通道中 ,检测到的电泳信号经过哈达玛反变换还原使信噪比提高 5倍 (理论上 5 .6倍 )的电泳谱 ,各组分的出峰时间、峰高和峰形均完全还原 ,毛细管电泳分离的采样频率不受影响 .
The first demonstration of Hadamard transform capillary electrophoresis(CE) is reported for enhancing the signal-to-noise ratios(S/N) on a microfluidic chip using a simple electrokinetically gated injection approach in this paper. Sample or running buffer was alternatively injected into the separation channel according to a 7-bit, 127-step pseudorandom binary sequence(PRBS). The electrophoregram rebuilt from detector signal with inverse Hadamard transform improved the S/N ratio by a factor of 5(5.6 in theory), compared to conventional single injection electrophoregram, almost without affecting the sample throughput.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2003年第10期1775-1778,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 0 2 990 3 0 )资助
关键词
微流控分析系统
芯片毛细管电泳
哈达玛变换
信噪比
数字信号处理
仪器分析
Microfluidic control analysis system
Chip-based capillary electrophoresis
Hadamard transform
Signal-to-noise ratio
Digital signal processing
