摘要
背景：近年来胃癌基因治疗的研究取得了一定的进展，但总体疗效尚不尽人意，目前正积极寻求组织特异性基因作为胃癌基因治疗的突破点。目的：以腺病毒为载体，研究p27kipl基因对胃癌细胞周期和DNA合成的影响。方法：将成功构建的携带人p27kipl基因的重组腺病毒载体Ad-p27kipl和LacZ重组腺病毒Ad-LacZ转染胃癌细胞系SGC-7901，并观察细胞形态的变化，流式细胞仪检测细胞周期和凋亡，^3H-胸腺嘧啶核苷(TdR)掺入实验测定细胞DNA合成。结果：Ad-p27kip1转染SGC-7901细胞后，细胞变圆、呈葡萄串样聚集以致脱落，G0／G1期细胞比例增加，8期、G2／M期细胞比例降低，并有凋亡发生，^3H-TdR掺入量亦显著降低。结论：腺病毒介导的p27kipl基因能使SGC-7901细胞产生G0／G1期阻滞，并能诱导细胞凋亡，抑制DNA合成，表明该基因疗法能有效抑制体外胃癌细胞的生长。
In recent years, the investigators have made some progress in the gene therapy of gastric cancer; however, the overall efficacy is not satisfactory. Tissue specific genes are sought to be the breakthrough point for gene therapy of gastric cancer at present. Aims: To investigate the effect of p27kipl gene on the cell cycle and DNA synthesis in gastric cancer cells by using adenovirus as the vector. Methods: The successfully constructed recombinant adenoviral vector Ad-p27kipl carrying human p27kipl gene and LacZ recombinant adenovirus Ad-LacZ were transfected to gastric cancer cell line SGC-7901. The morphologic changes of SGC-7901 cells were observed, the cell cycle and apoptosis were detected by flow cytometry, and DNA synthesis evaluated by ~3H-thymidine (TdR) incorporation, Results: After transfected with Ad-p27kipl, the SGC-7901 cells became round, botryoidal aggregate like, and exfoliating. The ratio of G0/G1 phase cells increased, while the ratio of S phase and G2/M phase cells decreased makedly. Apoptosis was found and ~3H-TdR incorporation decreased significantly. Conclusions: p27kipl gene mediated by adenovirus can cause blockage of G0/G1 phase cells, induce apoptosis and inhibit DNA synthesis in SGC-7901 cells, which indicate that this gene therapy can effectively inhibit the growth of gastric cancer cells in vitro.
出处
《胃肠病学》
2003年第4期210-214,共5页
Chinese Journal of Gastroenterology
