摘要
用超临界CO2对小麦胚芽脱脂,碱溶酸沉淀法提取麦胚蛋白,用8种蛋白酶分别进行单一酶和复合酶水解麦胚蛋白生产降血压肽,用高效毛细管电泳(HPCE)测定其活性。碱性蛋白酶生产水解物的活性最大,其对麦胚蛋白作用的最适pH为9,温度为50~55℃,加酶量为24AU/kg,水解度为16.5%时所得水解物对ACE有强烈抑制用。
After wheat germ was degreased by supercritical carbon dioxide ,the method of dissolving wheat germ protein byalkali and of deposition by acid was applied.The wheat germ protein was hydrolyzed by single and complex protease respec-tively in 8 kinds of proteases for antihypertensive peptide.Peptides activity was determined by high performance capillaryelectrophoresis. The hydrolyte obtained by alkaline protease showed the highest activity, with the optimum functionary pH as9 and temperature as 50~55℃,The quantity of protease used was 24AU/kg。
出处
《食品科学》
EI
CAS
CSCD
北大核心
2003年第10期120-123,共4页
Food Science
关键词
超临界CO2
小麦胚芽
麦胚蛋白质
降血压肽
蛋白酶
血管紧张素转化酶
酶解法
wheat germ protein
supercritical carbon dioxide
high performance capillary electrophoresis
angiotensoin-converting enzyme
antihypertensive peptides