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从麦胚蛋白质中制备降血压肽的研究 被引量:48

Study on Processing Antihypertensive Peptides Derived from Wheat Germ Protein
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摘要 用超临界CO2对小麦胚芽脱脂,碱溶酸沉淀法提取麦胚蛋白,用8种蛋白酶分别进行单一酶和复合酶水解麦胚蛋白生产降血压肽,用高效毛细管电泳(HPCE)测定其活性。碱性蛋白酶生产水解物的活性最大,其对麦胚蛋白作用的最适pH为9,温度为50~55℃,加酶量为24AU/kg,水解度为16.5%时所得水解物对ACE有强烈抑制用。 After wheat germ was degreased by supercritical carbon dioxide ,the method of dissolving wheat germ protein byalkali and of deposition by acid was applied.The wheat germ protein was hydrolyzed by single and complex protease respec-tively in 8 kinds of proteases for antihypertensive peptide.Peptides activity was determined by high performance capillaryelectrophoresis. The hydrolyte obtained by alkaline protease showed the highest activity, with the optimum functionary pH as9 and temperature as 50~55℃,The quantity of protease used was 24AU/kg。
出处 《食品科学》 EI CAS CSCD 北大核心 2003年第10期120-123,共4页 Food Science
关键词 超临界CO2 小麦胚芽 麦胚蛋白质 降血压肽 蛋白酶 血管紧张素转化酶 酶解法 wheat germ protein supercritical carbon dioxide high performance capillary electrophoresis angiotensoin-converting enzyme antihypertensive peptides
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参考文献5

  • 1Richard L.Soffer, Angiotensin-converting enzyme and the regulation of vasoactive peptides.Annu Rev Biochem, 1976,45:73-94.
  • 2Yamamoto N. Anfihypertensive peptides derived from food proteins.Biopolymers. 1997 ,43(2): 129-134.
  • 3Zhang R,Xu X,Chen T,Li Let aLAn assay for angiotensin-converting enzyme using capillary zone eleclxopHoresis Anal Biochem,2000 May 1,280(2):286-290.
  • 4Wu J,Aluko RE,Muir AD.Improved method for direct high-performance liquid chromatograpHy assay of angiotensin-converting enzyme-catalyzed reactions[J].J Chromatogr A 2002 Mar 15,950(1-2):125-130.
  • 5Jen Adler-Nissen.Deternination of the degree hydrolysis of food protein hyrolysates by trinrobenzenesulfonic acid[J].J Agric Food Chem,1979,27(6):1256-1262.

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