摘要
目的和方法 :为研究肾癌发生中参与的因子 ,用已知受p15调控的基因AK0 0 15 18作为诱饵筛选Gen Bank,然后用RT -PCR和Northern印迹实验方法 ,揭示p15与搜索出来的功能未知基因CCRG的关系 ,并探讨与肾癌发生的关系。结果 :得到 1个在氨基酸水平上与已知基因有 6 7%同源性的基因CCRG(GenBank登录号为XM-0 12 930 ) ;p15过量表达降低CCRG的表达 ;CCRG在肾癌组织和细胞中表达量明显高于正常肾组织和细胞。结论 :p15相关新基因CCRG在肾癌中高表达 ,可能是参与肾癌发生发展病理过程的重要基因。
AIM and METHODS: To analysis the factor that involved in renal carcin ogenesis, we used the bait gene AK001518 to screen GenBank. To understand the re lationship between cell cycle related gene(CCRG) and p15, we did RT-PCR and Nort hern Blot experiments. Th en we examined CCRG expression level in renal carcinogenesis. RESULTS: Gained a function unknown gene CCRG that was 67% a mino acid identical with the gene AK00 1 518 that was regulated by p15. It was shown that the CCRG mRNA was dramatically d ecreased when p15 gene was over-expressed. CCRG expression level was much higher i n tumor tissues and cells than normal tissues and cells. CONCLUSION: The novel g ene CCRG expressed highly in the renal carcinoma, which might play a significant role in the renal carcinogenesis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第10期1300-1304,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No :39970 36 9
30 0 70 70 3)
