摘要
目的 获得转基因菘蓝的分子生物学证据。方法 对经卡那霉素筛选的抗性再生菘蓝苗进行聚合酶链式反应 (PCR) ,Southern杂交和 RT-PCR检测。结果 PCR和 Southern杂交检测结果表明 ,外源半夏凝集素基因已经整合到转基因菘蓝的基因组中 ,RT-PCR结果表明其 DNA可以正常转录成 m RNA。结论 可以利用农杆菌介导的基因转化技术培育抗虫的菘蓝新品系。
Object To get molecular proof of transgenic Isatis indigotica Fort Methods The regenerated I. indigotica plantlets on kanamycin-containing media were anal ysed with polymerase chain reaction (PCR), Southern Blot and RT-PCR methods Results PCR and Southern Blot analysis showed that the foreig n PTA gene were inserted into the genome of the transformed I. indigotic a plants Further RT-PCR showed that the foreign PTA gene could be tra nscripted into mRNA normally. Conclusion Pest resistant I. indigotica can be obtained through Agrobacterium- mediated transformation
出处
《中草药》
CAS
CSCD
北大核心
2003年第9期846-849,共4页
Chinese Traditional and Herbal Drugs
