摘要
目的 :研究在大鼠老化过程中心钠素 ( ANF)基因的甲基化状态和该基因表达与调控的关系。方法 :采用限制性核酸内切酶 Hpa 和 Msp 消化大鼠基因组 DNA,用 [α- 3 2 P]d CTP进行ANF- c DNA标记探针 ,Southern blot杂交。结果 :老化过程中大鼠心房和心室 ANF基因内部至少存在一处未甲基化的序列。结论 :在老化过程中 ,大鼠心房和心室组织中的 ANF基因甲基化状态在转录水平上可能未参与该基因的表达调控 。
Objective:To study on the relationship between methylation of the ANF (Atrial Natriuretic Factor) gene and its expression regulation during aging. Methods:Using restriction endonuclease HpaⅡ,MspⅠdigest genomic DNA of the atria and ventricle and Southern blot hybridization with ANF cDNA probe by [α 32 P]dCTP marking was performed.Results:ANF gene was only partially methylation in atria and ventricle during rat′s aging, or saying remains 1 unmethylation CCGG seguence. Conclusion:Methylation status of the ANF gene of the atria and ventricle tissue may be not participate in expression and regulation of the ANF gene on transcription level during rat′s aging .The mechanism of the ANF gene expression and regulation needs further research. [
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第5期555-557,共3页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题 (392 70 732 )
