摘要
探讨神经节苷脂GM1对新生缺氧缺血性脑损伤大鼠神经的保护作用,本文对新生缺氧缺血性脑损伤(HIBD)大鼠用GM1治疗,并与假手术组和未治疗组对照,观察GM1对新生HIBD大鼠体重增长、脑重量、翻身、夹角尖叫等行为的影响,并通过大鼠游走水迷宫实验观察GM1治疗对其年长后学习和记忆的影响。结果显示:(1)实验后48h三组动物体重即已有差别,以HIBD组最低,对照组最高,随着日龄的增加体重的差别越来越明显。至42天以HIBD组体重最低,GM1治疗组和对照组相近;(2)对HIBD 48小时左右两脑半球重量测定显示,HIBD组右脑明显重于左脑,提示有脑水肿存在,72小时至42天,右脑重量则明显减轻,提示有脑萎缩存在;GM1治疗组48小时右脑也明显重于左脑,但72小时至42天两侧半球的重量无差异;(3)实验24小时内大鼠神经行为异常发生率以HIBD组最高,其次是GM1治疗组,对照组最低;48小时后GM1治疗组神经行为异常的发生率明显低于HIBD组,与对照组相比无差异。大鼠游走出水迷宫的时间以正常对照组游走有规律且时间短,GM1治疗组与正常对照组相比无统计学差异,而HIBD组大鼠走出迷宫时间较治疗组及对照组明显延长。结论:神经节苷脂GM1可以减少HIBD后的体重下降,使体重增长达正常水平。还可以减轻脑水肿和惊厥的发生,促进脑损伤的恢复。
To explore the neuroprotect effects of gangliosides GM1 to hypoxic ischemic brain damage (HIBD) newborn rats, HIBD model were made on 7 days wistar newborn rats, then the HIBD rats were therapied by GM1, the body weight, the weight of both hemisphere, cry after nipping tail, body turn over and water-maze procedure were used to research the development, hydrocephalus, neurobehavior and compare with the untreated HIBD rats and normal rats. The results show (1) from 48 hours to 42 days after HIBD the body weight of HIBD rats increased slowly than GMl treatment group and normal group, later two groups was similar. (2) 48 hours after HIBD the weihgt of right brain in HIBD group was more heavier than left side, which mean hydrocephalus present on right side. From 72 hours to 42 days after HIBD, right brain was lighter than left side, which means brain atrophy present. The right brains in GM1 treated group were also heavier than left side on 48 h after HIBD, they resume normal 72 h later. (3) the neurobehavior abnormal rate on HIBD group was highest on 24 hours after HIBD, GM1 treated group was lower, and normal group was lowest, on 72 hour HIBD group still higher than GM1 and normal group. A water-maze procedure for studing spatial learning show that HIBD rats need more time swiming to the bank than GM1 treated rats and normal rats on 42 days. Our conclusion is that gangliosides GM1 have neuroprotect effect to HIBD newborn rats, it can reduce brain edema and damage after HIBD, enhance learning and memoring ability.
出处
《新生儿科杂志》
2003年第4期163-167,共5页
The Journal of Neonatology
