摘要
从DNA水平上鉴定猴B病毒,并区别人单纯疱疹病毒(HSV-1);用PCR技术对猴B病毒和HSV-1进行扩增,并用限制性内切酶技术对扩增产物进行酶切,对PCR扩增产物和酶切产物进行电泳;电泳结果显示猴B病毒和HSV-1的PCR扩增产物为128bp,酶切后,B病毒能产生72bp和56bp片段,而HSV-1不能产生酶切产物;该方法能较好地鉴定猴B病毒,同时也将有密切抗原关系的HSV-1区分开来。
To develop a polymerase chain reaction (PCR) for detecting and distinguishing B virus from HSV-1. Suspected virus culture were detected for B virus DNA using TD-PCR method and the PCR products were examined by electrophoresis and restriction enzyme digestion. Result of PCR amplification of B virus and HSV-1 showed the same PCR products of 128 bp length, PCR products of B virus could be digested into two fragment (72 bp and 566 bp) by the restriction enzyme Sac II , but PCR product of HSV-1 couldn't produce that. TD-PCR method of B virus was developed successfully, and it could be an alternative technique for routine B virus diagnosis.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第A19期235-237,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广东省重点科技攻关资助项目(1997第35号)
