摘要
利用液体无机磷培养基,研究4种不同氮源硫酸铵、硝酸钠、尿素和硝酸铵对3种溶磷效率不同的溶磷真菌溶解宜昌产磷矿粉的结果表明,当只供应NH4+-N时,菌株P2.3的溶磷量最高,而当培养基中存在NO3--N时,菌株P66和P39的溶磷效率显著地高于只供应NH4+-N处理。对3种菌株不同氮源处理的培养滤液pH、有机酸含量和溶磷量三者之间的相关关系分析表明,培养滤液的pH值与有机酸含量呈高度负相关,而在不同氮源供给对溶磷量和培养滤液pH值和有机酸含量之间虽也有一定的相关性,但更主要的是与不同的氮源供应可能改变了不同溶磷真菌的代谢途径,使得产生有机酸种类及其含量发生了变化有关。3种溶磷真菌P2.3、P66和P39利用的最佳氮源分别是硫酸铵、硝酸钠和尿素,表明不同氮源供给对菌株的溶磷活性影响很大。图6,参10。
Effect of 4 different N sources (ammonium sulfate, sodium nitrate, urea and ammonium nitrate) on solubilization of rock phosphate which was produced in Yichang, Sichuan province by 3 different phosphate solubilizing fungal strains differed with phosphate solubih'zation activities was conducted in liquid media, the result showed that the strain of P2.3 had highest capacity to solubilize rock phosphate when supplied with NH4+ -N.However the strains P66 and P39 had higher phosphate solubih'zation activities with NO3--N than NH4+-N supply.To analysis the relationship among pH, organic acids and soluble P concentration of the culture filtrate indicated that pH value had highest negative correlation with organic acids concentration in the medium. Although a certain extent relationship between the soluble P concentration with pH and organic acids was confirmed in this experiment, more reasons for phosphate solubilization may be due to different N sources have changed both quality and quantity of organic acids produced by PSF.The best N sources for PSF strains P2.3, P66 and P39 to solubilize rock phosphate were ammonium sulfate, sodium nitrate and urea, respectively.This experiment demonstrated that different N sources supply has great effect on phosphate solubilization activities of PSF.
出处
《农业系统科学与综合研究》
CSCD
北大核心
2003年第4期260-263,共4页
System Sciemces and Comprehensive Studies In Agriculture
基金
国家"十五"科技攻关项目(2001BA007)
中国科学院东北地理与农业生态研究所学科前沿领域资助项目(KZCX3-SW-NA-04)
关键词
溶磷真菌
磷矿粉
氮源
溶磷
土壤微生物
phosphate solubilizing fungus
rock phosphate
N sources, phosphate solubilization
pH
organic acid
