摘要
目的探讨t(8;21)白血病干细胞克隆起源时间.实验方法用LD-PCR或LDI-PCR联合序列分析确定t(8;21)断裂区基因组DNA序列,根据所得序列设计病人特异性引物,用PCR方法检测患者Guthrie cards AML1-ETO融合基因;用RT-PCR检测脐带血AML1-ETO融合基因,阳性标本进一步用Real-time PCR进行AML1-ETO融合基因阳性细胞定量并用FISH检测用MiniFACS分取的CD33+和CD19+细胞组分t(8;21)易位.结果10例t(8;21)/AML患者中5例Guthrie cards AML1-ETO融合基因阳性;520份脐带血1份AML1-ETO融合基因阳性,脐血中AML1-ETO(+)细胞为1/2×104,仅CD33+细胞组份有t(8;21)易位.结论部分t(8;21)/AML患者其白血病干细胞克隆起源于胎儿期.
Object To investigate The origins of AML1 - ETO fusion gene positive leukemic stem cells. Methods AML1 - ETO genomic fusion sequences were characterized by LD - PCR/LDI - PCR combined with sequence, then the patients specific primers were designed for the detection of AML1 - ETO fusion gene of the patients' Guthrie cards by PCr; AML1 - ETO fusion gene expression of normal cord blood samples were determined by RT- PCr, the AML1 - ETO fusion gene positive cells in cord blood were qualified by real- time PCR; t (8; 21) translo-cation involved cell population were detected by FISH. Results Clonotypic genomic AML1 - ETO sequences were detected in the Guthrie spots for five individuals among the ten patients analysed; one AML1 - ETO fusion gene positive sample was found among a total of 520 normal cord bloods, the AML1 - ETO fusion gene positive cells in the positive sample was about 1/2×104, and t (8; 21) translocation just could been detected in CD33 + cells. Conclusion These data indicate that t (8; 21) positive leukemic stem cells can arise in ulero.
出处
《医学研究通讯》
2003年第9期7-9,共3页
Bulletin of Medical Research
基金
国家自然科学基金(30270573)
留学回国人员科研启动基金
