摘要
目的:研究基质金属蛋白酶-7(MMP-7)表达在胃癌发生和演进中的作用。方法:采用S-P免疫组化方法利用抗MMP-7、抗CD34抗体研究 113例胃癌原发灶中 MMP-7表达和微血管密度(MVD),比较 MMP-7 表达和 MVD与胃癌临床病理特征的关系,分析胃癌组织中MMP—7表达与MVD的关系。结果:MMP-7在胃癌旁黏膜中阳性表达率为29.2％(33/113),低于胃癌组织的阳性率 69.0％(78/113);MMP-7表达与胃癌肿块大小、浸润深度、转移和TNM 分期密切相关(P<0.05),而与胃癌的生长方式和分化程度无显著相关性(P>0.05);胃癌组织中MVD与肿块大小、浸润深度、转移和TNM分期关系密切(P<0.05),但与胃癌的组织分化和生长方式无显著相关性(P>0.05);特别是MVD的大小依赖于胃癌组织中 MMP—7表达(P<0.05)。结论:MMP-7在胃癌中表达上调,可作为揭示胃癌生物学行为的客观指标,其可能通过参与胃癌的生长、浸润、转移和血管形成而在胃癌的发生和演进中起到重要作用。
AIM: To investigate the role of matrix metalloproteinase-7 (MMP-7) expression in caricinogenesis and progression of gastric cancer. METHODS: We studied MMP-7 expression and microvessel density (MVD) in primary foci of 113 cases of gastric cancer by streptavidin-biotin-immunoperoxidase method with anti- MMP-7 and anti-CD34 antibodies. MMP-7 expression and mean MVD in primary foci were compared with clinico- pathological features of tumors, and the relationship be- tween MMP-7 expression and mean microvessel densities (MVD) in gastric cancer was concerned as well. RESULTS: MMP-7 showed positive expression in 29.2 % (33/113) of adjacent mucosa of gastric cancer was less than that in its primary foci (69.0 %, 78/113) of gastric cancer. MMP-7 expression in primary foci was closely cor- related with tumor size, invasive depth, metastasis and TNM staging of gastric cancer (P<0.05) despite no significant relationship between its expression and differentiation or growth pattern (P>0.05). Positive correlation of mean MVD with tumor size, depth of invasion, metastasis and TNM staging was found (P<0.05), but no relationship was found between mean MVD or differentiation of gastric cancer (P>0.05). Especially, mean MVD was dependent on MMP-7 expression in gastric cancer (P<0.05). CONCLUSION: MMP-7 is an effective marker to reveal the biological behaviors of gastric cancer and plays an important role in carcinogenesis and progression of gastric cancer by participating in growth, invasion, metastasis and angio- genesis.
出处
《世界华人消化杂志》
CAS
2003年第9期1310-1313,共4页
World Chinese Journal of Digestology
