摘要
目的:探讨中性粒细胞弹性蛋白酶(NE)抑制剂对细胞间黏附因子-1(ICAM-1)在大鼠胰腺移植物的表达及转导信号的影响.方法:采用大鼠胰十二指肠移植模型,实验组给予NE抑制剂(ONO-5046,10 mg/kg).体外实验检测NE及多种相关试剂对大鼠内皮细胞ICAM-1mRNA表达的影响及基因转导信号的调控作用.结果:对照组胰腺移植物中ICAM-1mRNA呈高水平表达,而实验组经ONO—5046处理后明显下调其表达,有显著性差异.NE刺激大鼠内皮细胞上调ICAM-1mRNA表达水平,而ONO-5046则明显抑制其表达;特异性钙离子载体增强核细胞的ICAM—1mRNA表达,相反,磷脂酶C抑制剂、钙离子螯合剂及核因子kB抑制因子则下调NE诱导的 ICAM—1mRNA表达水平.结论:NE增强ICAM-1在胰腺移植物的表达与细胞钙离子内流及磷脂酶C的信号转导有关.
AIM: To investigate the effect of neutrophil elastase inhibitor (ONO-5046) on expression of intercellular adhesion molecule-1 and transduction signal after pancreasduodenal transplan- tation in rats. METHODS: ONO-5 046 was injected intravenously into experimental animal models. ICAM-1 mRNA transduction signals were detected in rat endothelial cells with regard to the effect of many reagents on expression of ICAM. RESULTS: ICAM-1 mRNA level decreased in pancreatic grafts of experimental animals. ICAM-1 mRNA expression was increased in rat endothelial cells in vitro stimulated by NE, while that it could be inhibited by ONO-5046. Calcium ionophore enhenced ICAM-1 mRNA expression. In contrast, a phospholipase C inhibitor, calcium chelator and nuclear factor-kappa B inhibitor regulated down NE induction of ICAM-1 mRNA. CONCLUSION: ICAM-1 expression stimulated by NE in pan- creatic grafts may be associated with intracellular Ca^(2+) influx and a phospholipase C signal transduction.
出处
《世界华人消化杂志》
CAS
2003年第9期1396-1398,共3页
World Chinese Journal of Digestology
基金
辽宁省科委社发基金
No.99225003
辽宁省科委攻关课题资助项目
No.00225001
辽宁省教育厅高校科研项目
No.202012014
