摘要
目的:观察神经生长因子(nerve growth factor,NGF)和脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)基因修饰的嗅神经鞘细胞(Olfactory ensheathing cells,OECs)移植对损伤脊髓组织的保护作用。方法:将脊髓半横断伤SD大鼠模型,随机分为:NGF、BDNF基因修饰的OECs移植组(A组)、OECs移植组(B组)、损伤对照组(C组)和正常对照组(D组)。24h后每组8只动物取伤段标本,测水离子含量。其余动物第6周和12周每组8只动物爬坡试验,评价下肢运动功能及运动诱发电位(MEP)检测。结果:脊髓损伤(SCI)后组织水肿,Na^+、Ca^(2+)离子浓度升高,K^+、Mg^(2+)离子浓度降低。NGF、BDNF基因修饰的OECs脊髓内移植后显著改善这些变化,且使SCI后神经功能有显著恢复。结论:NGF、BDNF基因修饰的OECs脊髓内移植对SCI有保护作用。其机制可能与减少神经细胞离子失衡,改善细胞内环境有关。
Objective: To study the protective effects of the intracord transplantation of nerve growth factor(NGF) , brain-drived neurotrophic factor(BDNF) genes modified olfactory ensheathing cell(OECs) on spinal cord injury(SCI). Methods:Rats with semi-division(SD) of the spinal cord was divided into 3 groups. Group A consisted of the rat with SD treated with the transplantation of modified NGF,BDNF genes OECs,group B of the rats with SD treated with transplantation of OECs without genetic modification,group C of the rat with SD without treatment and group D was the normal control,24h after operation,8 rats of each group were killed and the injured segment of the spinal cord was resected to be examined with atomic absorption spectrophotometry. At 6 weeks and 12 weeks after transplantation, the anather rats were examined with neurological function test and MEP monitoring. Result:There was a significant increase of water content and Na+ ,Ca2+ ions and decrease of K+ , Mg2+ ions in the injured cord segment of group C and a statistically significant recovery was observed in group A. The intracord transplantation of modified OECs improved the neurological outcome of injured spinal cord. Conclusion:The intracord transplantation of NGF,BDNF genes modified OECs exerts protective effects on injured segment of spinal cord through the improvement of the internal ion enviroment of spinal cord.
出处
《中国矫形外科杂志》
CAS
CSCD
2003年第18期1277-1278,共2页
Orthopedic Journal of China
关键词
基因修饰
嗅神经鞘细胞
移植
脊髓损伤
Gentic modification
Olfactory ensheathing cell
Intracord transplantation
Spinal cord injury
