摘要
目的 探讨应用大剂量异丙基肾上腺素 (ISO)诱导大鼠心力衰竭模型的剂量和时限 ,以及如何提高动物存活率和模型成功率。方法 用不同剂量 (85mg kg ,170mg kg,340mg kg)ISO对大鼠进行皮下注射 2次 ,分别在不同时间段进行超声心动检查 ,测量左室舒张末径 (LVEDD) ,左室收缩末径 (LVESD) ,射血分数 (EF)。以EF <4 5 %作为心衰标准 ,来评估和鉴定模型的成功率 ,确定ISO应用的剂量和时限。结果 随着剂量的增大 ,模型成功的时间逐渐缩短 ,动物死亡率增加 (P <0 .0 1) ;随着时间的延长 ,动物心衰程度逐渐加重 ,死亡率增加 (P <0 .0 1) ;随着经验的积累 ,动物的急性死亡率可以降低。结论 ISO所致的心力衰竭有剂量和时间依赖性 ,采取必要的措施可以降低动物死亡率 ,提高模型成功率。
Objective To explore the dosage and time limit in application of isoproterenol(ISO) in rats with ISO-induced congestive heart failure(CHF) and h ow to improve the survival rate and successful rate of animals. Methods Rats received two subcutaneous injection of ISO which were separated by a 24-interval in different dosage.Cardiac function was assessed by echocardiogram at different time.The indexes include left ventricular (LV) end diastolic diameter (LVEDD),LV end systolic diameter (LVESD) and ejection fractio n (EF).The standard of CHF is EF<45%. Results Along with increased doses of ISO there was a decrease of t ime limit of a successful model,and an increase of mortality (P<0.01).As tim e go es on,the degree of CHF became more serious (P<0.01),the mortality was incre asing. Conclusion High doses of ISO produce a time-and dose-dependent impairment of cardiac function in CHF.And the acute mortality for the rat model of CHF is significantly improved with technical training and increasing of expe riences.
出处
《哈尔滨医科大学学报》
CAS
2003年第5期386-388,共3页
Journal of Harbin Medical University
基金
黑龙江省卫生厅资助项目 ( 2 0 0 2 0 3 6)
