摘要
目的:探讨肾毒血清性肾炎大鼠肾组织核因子-κB(NF-κB)活化及其意义。方法:肾毒血清肾炎应用免抗鼠肾小球基膜肾毒血清制备。应用凝胶电泳迁移率(EMSA)和Western blot检测肾毒血清肾炎大鼠肾组织中NF-κB活化及IκBα和IκBβ的降解;采用核酸酶保护法检测肾组织中IL-8表达,并分析其与NF-κB活化的关系。结果:模型组肾组织中IL-8表达显著高于正常对照组;肾毒血清肾炎大鼠肾组织中NF-κB活化显著增强,p65由胞质转移至胞核,胞质内IκBα和IκBβ降解明显增加;NF-κB活化与IL-8表达呈显著正相关。结论:NF-κB/IκB信号通路介导肾小球肾炎肾组织中IL-8表达。
Objective: To investigate the role of NF-KB/lKB signal pathway in the experimental nephrotoxic sera nephritis of rats. Methods: Nephrotoxic sera nephritis (NTN) was prepared by injection of anti-GBM antibody in rats. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-KB and degradation of 1KB. IL-8 expression was assessed by ribonu-clease protection assay. Results: NF-KB activation, nuclear translocation of p65 subunit, and degradation of iKBa and iKBpJ were significantly up-regulated in NTN rats compared to the control group, and so was the expression of IL-8. NF-KB activation was positively correlated with IL-8 expression. Conclusion: NF-KB /IxB signal pathway may play a pivotal role in pathogenesis of glomerulonephritis.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第6期533-535,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(30100081)
江苏省教育厅科研基金(99KJB320003)
