摘要
目的:进一步探讨姜黄素对LPS诱导的系膜细胞单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:采用不同浓度姜黄素处理体外培养的人肾小球系膜细胞,分别收集上清液及细胞,应用ELISA的方法测定上清液中MCP-1蛋白表达量,逆转录多聚酶链反应(RT-PCR)方法测定系膜细胞MCP-1基因的相对表达量。结果:LPS可显著上调系膜细胞MCP-1基因表达,同时刺激系膜细胞分泌MCP-1蛋白;姜黄素则呈浓度依赖性地抑制LPS诱导的系膜细胞MCP-1基因及蛋白表达。结论:姜黄素能抑制LPS诱导的肾小球系膜细胞MCP-1基因和蛋白的表达。
Objection:It has been shown curcumin inhibit the human mesangial cell growth and decrease its extracellular matrix release. This study was to determine whether curcumin inhibits the monocyte chemoattractant protein-1 (MCP-1) gene expression and MCP-1 protein release in human mesangial cells. Methods: Human mesangial cells were cultured with or without lipopolysaccharide (LPS,10 (Jig/ml) in presence or absence of various concention of curcumin (4, 16, 200 u,mol/L), collected the supernatant and the cells respectively. The levels of the MCP-1 protein in the supernatant were determined by means of ELISA after subconfluent quiescent mesangial cell incubated with various concentration of curcumin for 24 hours in vitro. And the MCP-1 mRNA expression were measured by semi-quantitative RT-PCR. Results: LPS could upregulate the MCP-1 mRNA expression and induce the MCP-1 protein release in human mesangial cells. Whereas curcumin could downregulate the mRNA expression and protein production of MCP-1 in mesangial cell induced by LPS in a dose-dependent manner. Conclusion: Curcumin could inhibit secretion of MCP-1 in mesangial cells induced by LPS and downregulate the mRNA expression of MCP-1 at the same time. The results suggest that curcumin may be profitable in curbing the inflammatory cells infiltrating in renal tissue and might be beneficial in retarding progressive development of glomerulonephritis.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第6期536-538,541,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省教育厅自然科学基金(01KJB320010)
南京市医学重点科技发展项目基金(ZKX0105)
关键词
姜黄素
系膜细胞
脂多糖
单核细胞趋化蛋白-1
curcumin
mesangial cell
lipopolysaccharide
monocyte chemoattractant protein-1
