摘要
目的 :观察牙本质基质蛋白 1(dentalmatrixprotein ,DMP1)在不同龋坏人牙齿中的表达 ,探讨DMP1在牙髓损伤修复中的作用。方法 :采用免疫组化SABC方法观察DMP1在不同龋坏人恒牙中的表达 ,并用图像分析系统进行半定量分析。结果 :DMP1在正常人恒牙组表达阴性 ,浅龋组部分成牙本质细胞和前期牙本质中弱阳性表达 ;中龋组成牙本质细胞数目增多 ,在胞浆和胞突以中阳性表达 ;深龋组成牙本质细胞层排列紊乱 ,出现空泡变性 ,成牙本质细胞样细胞形态细长、扁平 ,两种细胞胞浆和胞突中DMP1表达阳性 ,在修复性牙本质层强阳性表达。与对照组相比 ,成牙本质胞浆中DMP1含量在中龋组和深龋组明显增加 ,浅龋组无显著意义。结论 :DMP1参与成牙本质细胞样细胞分化分泌 。
Objective:To investigate the expression of dental matrix protein 1(DMP1)in carious human permanent teeth and its possible role during the reparative process after pulp injury.Methods:10 of noncarious third molars (NC),10 of shallow carious (SC),10 of intermediate carious (IC) and 10 of deep carious (DC) were prepared for the detection of DMP1 in odontoblasts by immunohistochemical staining. DMP1 expression in odontoblasts was analyzed by image analysis system and expressed as integral optical density (IOD).Results:DMP1 was majorly detected in cytoplasms and processes of odontoblasts and odontoblast-like cells.The IOD values in the groups of NC,SC,IC and DC were 0.265±0.018,0.309±0.021,0.678±0.031 * and 0.691± 0.033 * respectively;that in the reparative dentin in DC group was 0.856±0.045 ** ( *vs NC P< 0.05, **vs NC P<0.01).Conclusion: These results indicate that DMP1 is involved in the differentiation of odontoblast-like cells,as well as the formation of reparative and reactive dentin.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2003年第5期414-417,共4页
Journal of Practical Stomatology
关键词
牙本质基质蛋白1
DMPl
龋齿
免疫组化
SABC
Dental matrix protein 1
Dental caries
Immunohistochemistry
Image analysis system
