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中间普氏菌内毒素刺激前后下调人牙周膜细胞表达基因的研究

Effects of Prevotella intermedia lipopolysaccharide on the gene expression in periodontal ligment cells
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摘要 目的 :研究中间普氏菌内毒素刺激前后牙周膜细胞下调表达基因。方法 :应用点样数 5 12点的基因芯片分析中间普氏菌内毒素刺激前后牙周膜细胞下调表达基因。结果 :研究发现中间普氏菌内毒素刺激牙周膜细胞后 8条下调基因 ,包括转录调控基因、凋亡相关基因和受体基因。结论 :中间普氏菌内毒素刺激牙周膜细胞后 ,可引起部分基因表达下调 。 Objective:To study the gene expression in periodontal ligament cells (PDLCs) stimulated by Prevotella intermedia(Pi) lipopolysaccharide (LPS). Methods:PDLCs of passage 7 were treated with Pi LPS at 100 μg/ml for 24 h, then gene express of the cells was examined by a gene chip of 512 genes. Results: Genes of Z46973,M62810,AF109632,U45879,X81882,M10051,D14520 and L36529 were downregulated.Conclusion:Some genes in PDLCs can be downregulated by Pi LPS.
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2003年第5期462-465,共4页 Journal of Practical Stomatology
关键词 中间普氏菌 内毒素 牙周膜 基因芯片 脂多糖类 Prevotella intermedia Lipopolysaccharides, Gene chip Periodontal ligament cells
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