摘要
On the base of the characteristics of sulfate-reducing bacteria(SRB) growth and dish cultivated method, a new method for isolation and cultivation of SRB is established. It is dilution spread and repeat dish sandwish cultivated method with the exellence of dilution spread and embeded growth, and it can help separation and identification of SRB. The method has simple operation and less equipment. And using this method, the natural SRB drop was successfully gained, and small agar pieces containing SRB drop were acquired in easiness. The brief SRB transfering operation in strict anaerobic condition was indeed realized. Fig 1, Ref
On the base of the characteristics of sulfate-reducing bacteria(SRB) growth and dish cultivated method, a new method for isolation and cultivation of SRB is established. It is dilution spread and repeat dish sandwish cultivated method with the exellence of dilution spread and embeded growth, and it can help separation and identification of SRB. The method has simple operation and less equipment. And using this method, the natural SRB drop was successfully gained, and small agar pieces containing SRB drop were acquired in easiness. The brief SRB transfering operation in strict anaerobic condition was indeed realized. Fig 1, Ref 7
出处
《应用与环境生物学报》
CAS
CSCD
2003年第5期561-562,共2页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金 (2 0 0 760 30 )项目资助~~
关键词
厌氧菌
分离培养
稀释涂布
叠皿夹层
sulfate-reducing bacteria
isolation
dilution spread
repeat dish sandwish
