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牙周韧带细胞与成骨样细胞的体外共培养

Proliferation, protein synthesis and alkaline phophatase expression in co-cultured periodontal ligament cells and osteoblast-like cells
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摘要 目的 :探讨牙周韧带细胞 ( periodontalligamentcell,PDLC)和成骨样细胞的相互作用。 方法 :牙周韧带来源的PDLC和骨髓基质来源的成骨样细胞 (osteoblastlikecell,BC)进行体外套皿共培养 ,通过细胞增殖、蛋白质合成和碱性磷酸酶 (alkalinephosphatase ,ALPase)活性来分析细胞间相互作用。 结果 :PDLC较成骨样细胞增殖快 ,共培养条件对细胞增殖无显著影响 ;共培养对PDLC蛋白质合成没有影响 ,但对成骨样细胞蛋白质合成有影响 ;在共培养条件下 ,PDLC上调ALPase活性 ,成骨样细胞下调ALPase活性。结论 :在共培养体系中 ,PDLC和成骨样细胞存在相互作用 ,两者的分化趋势受到相互诱导。 Objective:To evaluate the cell-cell interaction between periodontal ligament cells and osteoblast-like cells.Method:PDLCs and osteoblst-like cells were co-cultured in vitro. Cell proliferation, protein synthesis and alkaline phosphatase expression was examined. Result:Co-culture system had no effect on cell proliferation, but the protein synthesis of PDLC. The ALPase activity is decreased in PDLCs while increased in osteoblast-like cells in co-culture system. Conclusion:PDLC and osteoblast-like cells exist cell -cell interaction that induce the cell differentiation each other.
出处 《临床口腔医学杂志》 2003年第11期670-672,共3页 Journal of Clinical Stomatology
关键词 牙周韧带细胞 成骨样细胞 共培养 碱性磷酸酶 periodontal ligament cell osteoblast-like cell alkaline phosphatase cell-cell interaction
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  • 1周彬,程祥荣,王贻宁,杨惠,熊卫星.纯钛表面牙周韧带细胞形成物的组织学和免疫组织化学研究[J].中国口腔种植学杂志,2001,6(3):113-115. 被引量:5
  • 2王培勇 刘健 许蜀国.介绍几种细胞共培养的方法[J].细胞生物学,1997,16(1):65-68.
  • 3King GN, King N, Cruchley AT, et al. Recombinant human bone morphogenetic protein- 2 promotes wound healing in rat periodontal fenestration defects[J]. J Dent Res, 1997 ,76(8) : 1460 - 70.
  • 4Lang H, Schuler N, Nolden R. Attachment formation following replantation of cultured cells into periodontal defects- a study in minipigs[J]. J Dent Res, 1998,77 (2):395-405.
  • 5Beresford JN. Osteogenic stem cells and the stromal system of hone and marrow[J]. Clin orthop, 1989,140(2) : 270 - 75.
  • 6Shimizu Y, Saito S, Tagami A, et al. Theartifificial resortation of dental root[J ]. J Oral Implantol, 1993,19(1 ) : 14 - 22.
  • 7Groeneveld MC, Everts V, Beertsen W. Loss of attachment in the marginal periodontium of the rat incisor under non- inflanmmtory conditions: expression of alkaline phosphatase activity [J ]. J Periodontal Res, 1996,31 (1) : 66- 72.

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