摘要
目的 通过研究鸡冠花黄酮类化合物对成骨细跑体外培养矿化作用和IGF -1表达的影响 ,探讨鸡冠花黄酮类化合物对大鼠成骨细胞的作用机理。方法 体外培养新生大鼠颅骨成骨细胞 ,茜素红染色方法 (ARS)研究对成骨细胞矿化的影响 ,SP免疫组化方法测定成骨细胞中IGF -1的表达情况 ,用HPIAS -10 0 0图象分析仪对各组表达进行定量分析。结果 鸡冠花黄酮类化合物促进大鼠成骨细胞矿化结节形成及IGF -1阳性表达 ,鸡冠花黄酮类化合物组的IGF -1平均光密度都显著高于阴性对照组 (P <0 0 5)。结论 鸡冠花黄酮类化合物可增强新生大鼠成骨细胞矿化和IGF
Objective To study the effect of flavonoid of celosia cri stata extract on mineralization and IGF-1 expression of cultured osteoblasts of rat. Methods The osteoblastic cells from cranlial bone of newb orn rat was cultured.The effect of mineralization was detected by alizarin borde aux stain(ARS).The effect of flavonoid of celosia cristata extract on IGF-1 expr ession of cultured osteoblasb was detected by SP method.The expression level was monitored with HPIAS-1000 photograph analysismeter.Results The IGF-1 of third cultured osteoblasts cultured used flavonoid of celosia cristata extract was positive expression.The IGF-1 po sitive expression of flavonoid of celosia cristata extract group was higher sign ificantly than negative control group ( P <0.05). Conclusion The flavonoid of celosia cristata extract may increase the mineralization and IGF-1 positive expression of cultured osteoblast s of rat.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2003年第11期1392-1393,共2页
Chinese Journal of Public Health
基金
河南省教育厅资助课题 ( 2 0 0 2 330 0 0 0 1 )
