摘要
的 研究高温对肿瘤细胞张力原纤维和微管纤维的影响。方法 将HeLa细胞分成 8组 ,置于不同温度 (3 7,40 ,43和 45℃ )下 ,分别经不同时间 (1h和 2h)水浴处理后 ,倒置显微镜下观察其形态变化 ,然后用考马斯亮蓝R2 50显示其张力原纤维 ,SABC法显示其微管纤维。结果 当温度由 40℃升至 43℃的过程中 ,首先引起HeLa细胞张力原纤维的解聚 ,进而引起微管纤维的解聚 ,随着温度的升高和时间的延长 ,两者的变化逐渐趋同。而微管组织中心具有较强的保护作用。结论 温和性高温对HeLa细胞张力原纤维的影响可能强于微管纤维 ,两者都呈现出渐进式的解聚变化 。
Objective To investigate the effect s of hyperthermia on tonofibrils and microtubules of tumor cells. Methods HeLa cells were divided into eight groups, placed respectiv ely in 37℃, 40℃, 43℃ and 45℃, incubated for 1h or 2hs. Inverted microscope w as used to observe its morphologic changes, Coomassie Blue R250 and SABC method were used to show its tonofibrils and microtubules. Results Hyperthermia led to depolymerization of tonofibrils of HeLa cells earlie r, then depolymerization of microtubules in the process of temperature ascending from 40℃ to 43℃.As the temperature decreases and the action time prolongs, bo th changes tend to become the same. The microtubule organizing center(MTOC) had powerful protection functions. ConclusionThe mild hyperthermia exerted stronger effects on tonofibrils than on microtubules; Under the hyperthermia, both tonofibrils and microtubules presented gradually changes of depolymerization, which closely related with cytotoxicity and apoptosis indu ced by heat.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2003年第11期658-661,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金资助项目 (N0 .59836 2 4 0 )
