摘要
介绍了RNA病毒拯救技术体系建立的简要过程、拯救成功与否的主要影响因素以及优化拯救体系的主要研究进展。着重介绍了该技术在分子病毒学研究和疾病防制中的重要应用及发展前景。
Full-length cDNA clone of RNA viruses,also named Reverse Genetics,is one of the most advanced techniques established in recent years,which plays a significant role on the development of molecule virology.The simple history of its construction,the affecting factors and optimizations of rescue system conditions were reviewed.More important,the valuable application and potential prospect of this technique in virology,prevention of diseases and related fields were mainly discussed.;
出处
《中国生物工程杂志》
CAS
CSCD
2003年第9期21-25,共5页
China Biotechnology
