摘要
流式细胞术是一种综合应用光学、机械学、流体力学、电子计算机、细胞生物学、分子免疫学等学科技术 ,对高速流动的细胞或亚细胞进行快速定量测定和分析的方法。它一秒钟能分析几千个细胞 ,并同时测定细胞的多个参数 ,广泛应用于生物医学的许多领域 ,如测定细胞的特征(形态、膜电位等 )和细胞内pH ,细胞DNA、蛋白质含量、表面受体、Ca2 + 等。对生物工程学来说 ,了解细胞的这些参数尤为重要 ,因为它们能比用传统技术测得的数据更好地描述细胞群体。从流式细胞仪对细胞多种参数的测定及原理 ,到它在生物工程学中的应用等方面进行了介绍 ,并讨论了流式细胞术的局限性和面临的挑战。
Flow cytometry is a general method for rapidly analyzing large numbers of cells individually using light-scattering,fluorescence and absorbence measurements.Flow cytometric assays have been developed to determine both cellular characteristics such as size,membrane potential,and intracellular pH,and the levels of cellular components such as DNA,protein,surface receptors,and calcium.Measurments that reveal the distribution of these parameters in cell populations are important for biotechnology,because they describe the population better than the average values obtained from traditional techniques.An overview of the principles of flow cytometry,with descriptions of methods used to measure various cellular parameters and examples of the application of flow cytometry in biotechnology is provided.Finally,a discussion of the challenges and limitations of the method is presented along with a future outlook.;
出处
《中国生物工程杂志》
CAS
CSCD
2003年第9期100-104,共5页
China Biotechnology
