摘要
目的 研究叶酸 (Folic Acid,FA)对高同型半胱氨酸 (HCY)小鼠血脂、心肝 MDA含量及心肌 SOD活性的影响 ,探讨防治心血管疾病的措施。 方法 昆明小鼠 2 4只 ,雄性 ,(2 0± 1) g,分 3组 :即正常对照组 (NS组 ) ,高同型半胱氨酸组 (HCY组 )与叶酸处理组 (FA组 )。 HCY动物造模给药途径为腹腔注射。 NS组给生理盐水 ,HCY组和 FA组给 HCY16μmol· kg- 1 d- 1 ;腹腔注射 2 h后 ,NS组和 HCY组经口灌胃给生理盐水 ,FA组给 FA 1.0μmol· kg- 1 d- 1 ,连续 5 d。末次给药后 4 h,腹腔静脉采血取血 ,分离脏器。测定血脂 ,心肝 MDA,心肌 SOD。 结果 HCY组与 NS组比 SOD活性有所升高 ,心肝 MDA、血浆 AL P含量增加 ,差异显著。FA组与 HCY组比心肝 MDA和血浆 AL P含量显著降低。与 NS组比 HCY组动物的 TG、TC、L DL、VL DL含量显著增高 ,HDL 显著下降 (P<0 .0 5 ) ;FA组与 HCY组比 TG、TC、L DL、VL DL含量显著下降 ,HDL显著增高 (P<0 .0 5 )。 结论 叶酸作为一种细胞的保护因子在临床应用可抵抗高同型半胱氨酸引起的高血脂和脂质氧化 。
Objective To study the effect of Folic Acid(FA) on plasma lipids, MDA and SOD activity in HCY mice, and try to find out the measure to control cardiovascular disease. Methods Twenty four male kunming mice were randomly divided into three groups: control group (0.1ml Saline injected in abdominal cavity), HCY group and FA group (HCY 16μmol·kg -1 d -1 ); Intragastric administration after 2h of abdominal cavity injection: NS group and HCY group (0.1ml Saline), FA group ( folic acid 1.0μmol·kg -1 ). This was done for a ontinuous of 5 days. 4h After the last administration, get blood from abdominal cava. The content of plasma TG,TC,HDL,LD,VLDL,ALP and DMA of liver and heart, and SOD activity of heart were measured. Result Compared with the NS group, MDA, SOD, plasma TG,TC, LD, VLDL, ALP were increased significantly in HCY group, but the plasma HDL was decreased; Compared with the HCY group, MDA, plasma TG,TC, LD, VLDL in FA group was significantly decreased. Conclusion Folic acid may resist the high blood lipids and decrease the lipid peroxidation, and prevent the injury of liver and heart in HCY mice.
出处
《实用预防医学》
CAS
2003年第5期627-629,共3页
Practical Preventive Medicine
基金
河南省自然科学基金项目 (0 1 2 4 1 70 745)
