摘要
目的 研究氟牙症的发生机制。方法 选择 2 0只Wistar大鼠 ,随机分为 2组 :Ⅰ组 (对照组 )和Ⅱ组 (5 0mg/LF-)。 8周后处死动物 ,利用磨片、HE染色、核仁形成区嗜银染色 (AgNORs)和原位细胞凋亡检测 (TUNEL)技术观察过量氟对大鼠切牙形态及机能的影响。结果 Ⅱ组大鼠切牙釉质生长线明显 ,分泌期成釉细胞嗜伊红颗粒蓄积 ,分泌前期成釉细胞AgNORs颗粒数低于Ⅰ组 ,差异有显著性 (P <0 0 0 1) ,成釉细胞凋亡增多 ,并伴有凋亡现象向分泌期迁移。结论 过量氟可抑制成釉细胞增生 ,促进成釉细胞凋亡 。
Objective To observe the effect of overdose fluoride on the proliferation of rat′s incisor ameloblast Methods 20 Wistar rats were divided randomly into 2 groups: Group Ⅰ (Control);Group Ⅱ 50 mg/L F - were given After 8 weeks treatment, the AgNORs stain and TUNEL technique were applied to analyze the effect of fluoride on the proliferation and apoptosis of ameloblasts Results The imagination analysis results showed that proliferation of pre secretion ameloblasts were inhibited in group Ⅱ as compared with the control group( P <0 001) There was significant increase of apoptosis with the trend of migration toward secretion stage Conclusion The mechanism of fluorosis mottled enamel may be the effect of overdose fluoride with inhibits proliferation and induces apoptosis of ameloblasts resulting in dysfunction of secretion or absorption of enamel matrix proteins
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2003年第3期217-219,I005,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金资助项目(3 973 0 3 90 )
