摘要
目的 :观察熟地黄对动物学习记忆障碍的影响及机理。方法 :采用氯化铝 (AlCl3)拟痴呆小鼠模型和谷氨酸单钠 (monosodiumglutamate ,MSG)毁损下丘脑弓状核大鼠模型 ,通过跳台实验和Morris水迷宫实验观察熟地黄对学习记忆的影响 ;薄层扫描测定脑组织谷氨酸 (glutanateacid ,Glu)和γ 氨基丁酸 (γ aminobutyricacid ,GABA )含量 ;免疫组化观察大鼠海马N 甲基 D 门冬氨酸受体 1(N Methyl D AspartateReceptorone ,NMDAR1)和GABA受体 (GABAR )在海马的表达。结果 :熟地黄能延长痴呆动物跳台实验潜伏期、减少错误次数 ;缩短水迷宫实验寻台时间 ;调节痴呆模型动物脑Glu和GABA含量 ,提高NMDAR1,GABAR在海马的表达。结论 :熟地黄有改善AlCl3 小鼠和MSG大鼠学习记忆作用 ,作用机理可能与调节脑Glu和GABA含量 ,提高MSG大鼠NMDAR1,GABAR在海马的表达有关。
Objective: To observe the mechanism of SHU -Dihuang on the function of lea rning and memory. Method: On the dementia model mouse caused by A lCl 3 and the rats model damaged thalamic arcuate nucleus with MSG, we observed t he function of learning and memory by step down task and Morris water maze task, mensurated the content of glutamic acid and γ-aminobutyric acid by TLC,and o bs erved the expression of NMDAR1 and GABAR in hippocampi by immunohistochemical me ans. Result: Shu Di Huang could decrease the times of mis takes and prolong the incubation period in step down task, and shorten the incubation period of seekin g the platform in Morris water maze task. Shu Di Huang could adjust the content of Glu and GABA in brain, and increase the expression of hippocampal NMDAR1 and GABAR as well. Conclusion: Shu Di Huang can improve the function of learning and memory of dementia animal model,and its mechanism may be related to the adjust ment of the content of Glu and GABA in brain, and increase of the expression of hippocampal NMDAR1 and GABAR.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2003年第9期862-866,共5页
China Journal of Chinese Materia Medica
