摘要
目的 对归脾片剂 3味药材的TLC同板一次性薄层鉴别 ,并对制剂中的指标性成分甘草酸进行HPLC建立和含量测定。方法 采用TLC对党参、远志、甘草进行同时色谱鉴别 ,HPLC测定甘草酸含量。结果 鉴别方法简便快速。甘草酸相关系数r =0 .9999,回收率为 98.6 7% ,RSD为 0 .5 4 %。结论 建立的方法可有效地控制该片剂的质量。
OBJECTIVE To identify the three crude medicinal materials and determine the content of glycyrrhizic acid in Guipi tablet.METHOD The simultaneous TLC and HPLC methods were applied to identification and quantitative analysis, respectively.RESULTS The TLC was simple and rapid method for identification The HPLC assay was linear over the range of 0.2-1.0 mg/mL( r =0.999).The average recovery and the intra day precision of HPLC were 98.67% and 0.54%(RSD), respectively.CONCLUSION The methods developed were effective for control the quality of Guipi preparation.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2003年第5期376-378,共3页
Chinese Journal of Modern Applied Pharmacy
关键词
归脾片
党参
远志
甘草
TLC
HPLC
Guipi preparation
Radix Codonopsis
Radix Polygalan
Radix Glycyrrhizae
TLC
HPLC
