摘要
目的 :以IL 1β所致胃溃疡复发大鼠为模型 ,从胃黏膜上皮细胞凋亡以及Bcl 2、Bax和MK表达方面 ,探讨健胃愈疡颗粒抗胃溃疡复发的分子生物学机理。方法 :Okabe乙酸法制作大鼠胃溃疡模型 ,ipIL 1β制作复发模型。观察胃组织病理学变化 ,检测胃黏膜上皮细胞凋亡、Bcl 2、Bax和MKmRNA表达。结果 :健胃愈疡组溃疡复发率、凋亡指数 (AI)明显降低 ,Bcl 2、Bcl 2 Bax、MKmRNA表达明显高于模型复发组。结论 :健胃愈疡颗粒可增加Bcl 2及MK的表达 ,减少胃黏膜上皮细胞凋亡 ,降低IL 1β所致的大鼠胃溃疡复发。
Objective:To investigate the molecular biological mechanism of JWYY preventing Gastric ulcer (GU) relapse,We studied the effect of JWYY on the epithelial cell apoptosis and the expression of Bcl 2,Bax and MK in gastric ulcer recurrence model on rats caused by IL 1β.Methods:GU model was induced by acetic acid described by Okabe.Ecurrence model were administered IL 1β.Observation included:pathological examination,gastric ulcer index (GUI),gastric mucosal epithelial cell apoptosis;the expression of Bcl 2,Bax and MK mRNA.Results:JWYY granule can increase the expression of Bcl 2 protein and mRNA and the ratio of Bcl 2/Bax in gastric mucosa,reduce epithelial cell apoptosis and ulcer relapse.Conclusion:JWYY granule can increase the expression of Bcl 2 and MK mRNA in gastric tissue,reducing epithelial cell apoptosis,so prevent ulcer relapse caused by IL 1β.
出处
《中国中医基础医学杂志》
CAS
CSCD
2003年第9期25-27,共3页
JOURNAL OF BASIC CHINESE MEDICINE
