摘要
对玉米灰斑病菌孢子产生、病菌接种和寄主抗病性测定技术的研究结果表明,应用玉米叶粉碳酸钙琼脂和玉米叶粉琼脂两种培养基,温度24～25℃,培养5天,病菌可大量产生分生孢子;于植株喇叭口期,用注射器将病菌孢子悬浮液注射于植株喇叭口中,获得了理想的发病效果;对20个玉米自交系注射接种鉴定结果表明,玉米自交系间抗病性差异明显,但未发现免疫自交系。
Conidial production, inoculation and the pathogenicity test of Cercospora zeae-maydis Tehon & Daiels on maize were studied. The result showed that the pathogen on medium of maize leaf powder agar (MLPA) or maize leaf powder plus CaCO3 agar (MLPCA) , cultured 5 days at 24 -25℃, was found to produce abundant quantities of conidia. Inocula was inoculated into leaf whorl of plants by a hypodermic syringe at 9 - 11 leaf stage of plant. The increased disease incidence was observed in the fields. Identification of 20 inbred of maize for disease resistance showed that there was significantly different resistance to Cercospora zeae-maydis, but none was immune to the disease.
出处
《植物保护学报》
CAS
CSCD
北大核心
2003年第2期129-132,共4页
Journal of Plant Protection
基金
辽宁省博士启动基金(2001102064)
关键词
玉米
灰斑病
接种技术
抗病性鉴定
gray leaf spot of maize, technique of inoculation, identification of resistance
