摘要
目的 研究抗氧化剂 N-乙酰半胱氨酸对全脑缺血诱导海马组织信号转导与转录激活子 - 3(STAT3)的激活及 DNA结合活性的影响。方法 采用 SD雄性大鼠四动脉结扎 (4- VO)全脑缺血模型 ,以及腹腔注射给药的方法。运用抗磷酸化 STAT3抗体做免疫印迹 (IB)检测海马核抽提物磷酸化 STAT3的变化 ;以及电泳迁移率改变实验 (EMSA)分析核内 STAT3DNA结合活性的变化。结果 全脑缺血不同时间导致核内 STAT3磷酸化水平及 DNA结合活性的持续增高 ;缺血前 2 0 m in腹腔注射抗氧化剂 N-乙酰半胱氨酸能明显抑制其增高。结论 全脑缺血所致 STAT3的磷酸化水平及 DNA结合活性的增高与氧化应激有一定的关系。
Objective To investigate the influence of N-acetyl cysteine on the changes of activation of STAT3 and DNA binding activity in hippocampus after global brain ischemia. Methods Four-vessel occlusion ischemia model of SD rats was used in this study. The changes of p-STAT3 in nuclear extracts were assessed by immunoblotting(IB) using special anti-p-STAT3 antibody(Tyr 705) and electrophoretic mobility shift assay(EMSA) was used to analyze DNA binding activity of STAT3. Results p-STAT3 and DNA binding activuity were continuously increased during ischemia in hippocampus and were inhibited by N-acetyl cysteine,which was administered to the rats by abdominal injection 20 minutes before occlusion. Conclusion The increments of p-STAT3 and DNA binding activity following cerebral ischemia are inhibited by N-acetyl cysteine,which indicating oxidative stress is involved in the process of activation of STAT3 induced by ischemia.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2003年第5期391-393,共3页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金资助项目 (No. 3 0 0 70 182 )
关键词
N-乙酰半胱氨酸
脑缺血
酪氨酸磷酸化
信号转导与转录激活子-3
DNA结合活性
Cerebral ischemia
Signal transducer and activator of transcription-3(STAT3)
N-acetyl cysteine
Tyrosine phosphorylation
Electrophoretic mobility shift assay(EMSA)
