摘要
以肌动球蛋白的盐溶性、肌原纤维蛋白ATPase活性以及肌原纤维蛋白的巯基含量为指标,研究了鳙肌原纤维蛋白在-10℃,-20℃,-30℃和-40℃下冻藏时的变性情况。结果表明,在不同温度下冻藏时,鳙肌动球蛋白的盐溶性、肌原纤维蛋白的ATPase活性以及巯基含量随着冻藏时间的延长,均呈下降趋势。但是,鳙蛋白质的变性速度在不同冻藏温度下的差异是极其显著的(P<0.01)。冻藏温度越低,变性越缓慢。
The denaturation of myofibrillar protein of bighead carp (Aristichthys nobilis) during frozen storage at different temperatures(-10℃,-20℃,-30℃ and -40℃) was studied by measuring the solubility of actomyosin, ATPase activities and -SH content of myofibrillar protein. The results indicated that the solubility of actomyosin, ATPase activities and -SH content of myofibrillar protein decreased as frozen storage progressed. The temperature of frozen storage can significantly influence the rate of denaturation of myofibrillar protein (P<0.01). The lower the frozen storage temperature, the slower the rate of denaturation of protein is.
出处
《水产学报》
CAS
CSCD
北大核心
2003年第5期480-485,共6页
Journal of Fisheries of China
基金
山东省攻关课题(003110107)
关键词
鳙
冻藏
肌原纤维蛋白质
变性
Aristichthys nobilis
frozen storage
myofibrillar protein
denaturation
