摘要
细胞表面工程 ,又称GPI锚定蛋白转移法 ,是一种不依赖基因转移而能够在细胞膜上表达新蛋白的有效方法。GPI锚定蛋白可以通过其尾部脂质GPI结构自然地整合到细胞膜上。这种方法克服了基因转移技术的一些局限 ,在临床应用上更具优势。该技术可用于肿瘤疫苗和肿瘤免疫治疗方面的研究 ,将GPI锚定修饰的免疫分子直接转移到肿瘤细胞膜上 。
Cell-surface engineering technique,also named as GPI-anchored protein transfer, has become an attractive strategy to express new proteins on cell membranes without gene transfer. This method involves purified glycosyl-phosphatidylinositol (GPI)-anchored proteins which can spontaneously incorporate their lipid tail into cell membranes. This technique has overcome several of the limitations of gene transfer techniques and thus offers many advantages in consideration of human clinical trials. The applications of GPI-anchored protein transfer include its use for the development of cancer vaccines and cancer immunotherapy. Direct modification of tumor cell membranes by transfer of a glycosyl-phosphatidylinositol (GPI)-anchored immunological molecules has been proposed as a novel potentially useful strategy to development of new type of therapeutic tumor vaccines.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第6期478-479,481,共3页
Immunological Journal
基金
国家"8 6 3"课题资助项目 (2 0 0 1AA2 17131)
