摘要
本文研究了EGF、PTH和RA对UMR106细胞EGF受体的调节作用。结果显示PTH能上调EGF的受体,UMR106细胞经bPTH(1-34)处理3天,EGF受体的相对结合率与对照比较提高了40.3％,每个细胞的EGF受体数目从7.22×10~3增加到1.44×10~4,Kd从2.02×10^(-11)增加到3.68×10^(-11)mol/L。而RA则能下调EGF受体,以RA处理3天,EGF受体数目从7.22×10~3下降到4.28×10~3,Kd则从2.02×10^(-11)增加到4.17×10^(-11)mol/L。提示PTH和RA可能通过调变其EGF受体而分别起到正性和负性生长调节作用。
Rat osteoblastsarcome UMR 106 cell line has been shown to posses specific receptors for epidermal growth factor (EGF), parathroid hormone (PTH) and retinoic acid (RA).Using radioreceptor assay, the presence of EGF and PTH receptors in UMR 106 cells was established.Their specific binding rate is 11.75 percent and 14.49 percent, respectively.In these studies, we examined the influence of pre-treament PTH, EGF and RA on EGF receptor of UMR 106 cells which resulted in significant regulation of the specific binding of EGF receptors.Cells were pre-treat ed with bPTH (1-34) (2********x10-12-2*********x10-8mol/L) for 3d, the relative binding of 125I-EGF to its receptors increased as a function of dose.At 2********X10-8mol/L of PTH,the relative binding of 125I-EGF increased 40.3% as compare to the control.While the relative binding of 125I-EGF decreased to 47% of the control after treatment of RA (10-5mlo/L) for 3d.Scatchard analysis of control and bPTH pre-treated cells showed increased available EGF binding site from 7.22*********x103 to 1.44********x104 receptors per cell| and increased receptor apparent Kd from 2.02**********X10-11 to 3.68********x10-11mol/L.In contf-ast, RA treatment decreased available EGF binding site from 7.22*********X103 to 4.28**********x103 receptors per cell, and its apparent Kd was also increased to 4.17******** X10-11mol/L.These data suggest that EGF receptors in UMR 106 cell were up and down regulated by PTH and RA respectively, and therefore PTH and RA could be the potent regulators of cell growth and proliferation, through EGF receptors.
基金
生物膜与膜生物工程国家重点实验室资助
关键词
EPF
BA
成骨肉瘤
甲状旁腺激素
Rat Osteoblastsarcoma Cell Line, Parathroid Hormone, Epidermal Growth Factor
Retinoic Acid, Receptors
