摘要
以欧美杨108为试材,对组培苗叶片、茎段进行初步的转化受体系统研究和对选择性抗生素卡那霉素的敏感性测定,为杨树的基因转化及转化选择提供基础。通过实验认为,以叶片与茎段为转化受体材料,虽然茎段诱导率高于叶片,但叶片长势上要好于茎段,叶片诱导率能达到转化的要求;叶背不宜为受体材料,叶片伤口不宜过大;腋芽的存在与否,在诱导率上没差别,都可达100%,但腋芽有利茎段分化和分化芽的生长。从多个角度来分析用于筛选转化苗的卡那霉素下限浓度,以叶片为转化受体时,为30mg/L;以茎段为转化受体时,为50mg/L;生根阶段,下限为30mg/L。
Taking Populus euramericana cv. '114/69' as testing material, studies on transformation acceptor systems of leaves and stems of tissue cultural seedlings were carried out, and sensitivity of selective antibiotic - kanamycin was determined, which lay a foundation for gene transformation and transformable selection of poplar. The results revealed that: taking leaf and stem as transformation acceptor material, although induced rate of stem was higher than leaf, leaf grew better than stem and induced rate of leaf might meet the requirements of transformation. Reversed leaf was not suitable to be as acceptor material, and cut of leaf should be not so big; there was no difference for induced rate whether axillary bud existed, induced rate might reach 100%, but axillary bud was favorable for splitting of stem and growth of bud. Analyzing from many angles, lower limit concentration of kanamycin used for selection of transformation seedlings was 30mg·L-1 and 50 mg·L-1 when taking leaf and stem as acceptor, respectively; at rooting stage, lower limit concentration was 30mg·L-1.
出处
《辽宁林业科技》
2003年第5期9-10,18,共3页
Liaoning Forestry Science and Technology
关键词
受体系统
卡那霉素
杨树
acceptor system, kanamycin, poplar
