摘要
目的 研究红豆杉细胞提取物对肝癌细胞 SMMC- 772 1的生长抑制作用及其机制。方法 运用台盼蓝拒染法测定肿瘤细胞生长曲线 ,流式细胞仪和 Giem sa染色分析肿瘤细胞周期和细胞凋亡。结果 红豆杉细胞二氯甲烷提取物对肝癌细胞 SMMC- 772 1的生长有明显的抑制作用 ,流式细胞仪分析发现 G2 / M期肿瘤细胞数量增多 ,经 Giem sa染色发现肿瘤细胞有凋亡发生。结论 红豆杉细胞提取物可以通过诱导细胞发生凋亡而抑制肝癌细胞SMMC- 772
Object To study the inhibitory effect and mechanism of extract from cultured cell of Taxus chinensis (Pilg.) Rehd. on hepatocarcinoma cell line SMMC 7721. Methods Trypan blue stain assay was used to determine tumor cell growth curve, flow cytometry and Giemsa staining were used to analyze cell cycle and cell apoptosis. Results Inhibitory effect happened on tumor cell line SMMC 7721, G 2/M stage of tumor cells increased with the addition of T. chinensis cell extract by carrene and apoptosis happened. Conclusion The extracts from cultured cell of T. chinensis have inhibitory effect on SMMC 7721 cells and can further induce cell apoptosis.
出处
《中草药》
CAS
CSCD
北大核心
2003年第11期1013-1015,共3页
Chinese Traditional and Herbal Drugs
基金
"九五"国家攻关项目 (96- C0 2 - 0 3- 0 1 )
关键词
红豆杉细胞
SMMC-7721
细胞增殖
细胞凋亡
cell of Taxus chinensis (Pilg.) Rehd.
SMMC 7721
cell proliferation
cell apoptosis
